Biological applications of microchip electrophoresis with amperometric detection: in vivo monitoring and cell analysis.
Electrochemical detection
Electrophoresis
Microdialysis
Neurotransmitters
Nitric oxide
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
16
02
2020
accepted:
06
04
2020
revised:
29
03
2020
pubmed:
30
4
2020
medline:
4
5
2021
entrez:
30
4
2020
Statut:
ppublish
Résumé
Microchip electrophoresis with amperometric detection (ME-EC) is a useful tool for the determination of redox active compounds in complex biological samples. In this review, a brief background on the principles of ME-EC is provided, including substrate types, electrode materials, and electrode configurations. Several different detection approaches are described, including dual-channel systems for dual-electrode detection and electrochemistry coupled with fluorescence and chemiluminescence. The application of ME-EC to the determination of catecholamines, adenosine and its metabolites, and reactive nitrogen and oxygen species in microdialysis samples and cell lysates is also detailed. Lastly, approaches for coupling of ME-EC with microdialysis sampling to create separation-based sensors that can be used for near real-time monitoring of drug metabolism and neurotransmitters in freely roaming animals are provided. Graphical abstract.
Identifiants
pubmed: 32347360
doi: 10.1007/s00216-020-02647-z
pii: 10.1007/s00216-020-02647-z
pmc: PMC8130646
mid: NIHMS1589059
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
6101-6119Subventions
Organisme : NINDS NIH HHS
ID : R21 NS061202
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32GM008545
Pays : United States
Organisme : National Science Foundation
ID : CHE -1411993
Organisme : NIA NIH HHS
ID : P30 AG035982
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008545
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS042929
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103638
Pays : United States
Organisme : NINDS NIH HHS
ID : R21NS061202
Pays : United States
Organisme : NINDS NIH HHS
ID : R43 NS064644
Pays : United States
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