MiR-543 regulates myoblast proliferation and differentiation of C2C12 cells by targeting KLF6.
KLF6
differentiation
miR-543
myogenesis
proliferation
Journal
Journal of cellular biochemistry
ISSN: 1097-4644
Titre abrégé: J Cell Biochem
Pays: United States
ID NLM: 8205768
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
20
08
2019
accepted:
12
02
2020
medline:
30
4
2020
pubmed:
30
4
2020
entrez:
30
4
2020
Statut:
ppublish
Résumé
MicroRNA-543 (miR-543) has been found to play a suppressive role in various human cancers in many studies, whereas the specific functions of miR-543 in muscle development remain poorly understood. Here, we found that the expression of miR-543 was high in skeletal muscle and increased during the differentiation of C2C12 cells. Overexpression of miR-543 repressed C2C12 cell proliferation and promoted differentiation, while knockdown of miR-543 expression produced the opposite results. During myogenesis, we predicted and verified that Krüppel-like factor 6 (KLF6), a suppressor of multiple tumor cells, was a target gene of miR-543. Then, miR-543 was found to specifically target KLF6 and repress its expression. Besides this, knockdown of KLF6 promoted the differentiation but inhibited the proliferation of C2C12 cells. Si-KLF6 can rescue the influence of miR-543 inhibitor on C2C12 cell differentiation. Our results indicate a new regulatory mechanism of miR-543 on KLF6 expression and suggest the possibility of using the miR-543/KLF6 pathway as a potential target for studying myogenesis.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4827-4837Subventions
Organisme : National Porcine Industry Technology System
ID : CARS-35
Informations de copyright
© 2020 Wiley Periodicals, Inc.
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