Evaluation of Single-Cell Cytokine Secretion and Cell-Cell Interactions with a Hierarchical Loading Microwell Chip.
T cell function
adoptive cell therapy
cell-bead pairing
cell-cell interaction
cytokine detection
cytotoxicity
microfluidics
single cell
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
28 04 2020
28 04 2020
Historique:
received:
15
10
2019
revised:
21
02
2020
accepted:
02
04
2020
entrez:
30
4
2020
pubmed:
30
4
2020
medline:
14
5
2021
Statut:
ppublish
Résumé
Comprehensive evaluation of single T cell functions such as cytokine secretion and cytolysis of target cells is greatly needed in adoptive cell therapy (ACT) but has never been fully fulfilled by current approaches. Herein, we develop a hierarchical loading microwell chip (HL-Chip) that aligns multiple cells and functionalized beads in a high-throughput microwell array with single-cell/bead precision based on size differences. We demonstrate the potential of the HL-Chip in evaluating single T cell functions by three applications: high-throughput longitudinal secretory profiling of single T cells, large-scale evaluation of cytolytic activity of single T cells, and integrated T cell-tumor cell interactions. The HL-Chip is a simple and robust technology that constructs arrays of defined cell/object combinations for multiple measurements and material retrieval.
Identifiants
pubmed: 32348757
pii: S2211-1247(20)30523-4
doi: 10.1016/j.celrep.2020.107574
pmc: PMC7583657
mid: NIHMS1589038
pii:
doi:
Substances chimiques
Cytokines
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
107574Subventions
Organisme : NCI NIH HHS
ID : U54 CA210181
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA035868
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA101795
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI127704
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG052507
Pays : United States
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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