Multimodal Targeted Deep Sequencing of Circulating Tumor Cells and Matched Cell-Free DNA Provides a More Comprehensive Tool to Identify Therapeutic Targets in Metastatic Breast Cancer Patients.

cell-free DNA circulating tumor DNA circulating tumor cells liquid biopsy metastatic breast cancer patients multi-parametric multimodal next-generation sequencing therapy stratification unique molecular indices

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
27 Apr 2020
Historique:
received: 23 03 2020
revised: 17 04 2020
accepted: 22 04 2020
entrez: 1 5 2020
pubmed: 1 5 2020
medline: 1 5 2020
Statut: epublish

Résumé

Cell-free DNA (cfDNA) and circulating tumor cells (CTCs) exhibit great potential for therapy management in oncology. We aimed to establish a multimodal liquid biopsy strategy that is usable with minimized blood volume to deconvolute the genomic complexity of metastatic breast cancer. CTCs were isolated from 10ml blood of 18 hormone receptor-positive and human epidermal growth factor receptor 2-negative (HER2-) metastatic breast cancer patients. cfDNA was isolated from plasma generated after CTC depletion and targeted sequencing analyses were conducted.

Identifiants

pubmed: 32349306
pii: cancers12051084
doi: 10.3390/cancers12051084
pmc: PMC7281124
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : QIAGEN GmbH
ID : N/A
Organisme : Graduate School of Biomedical Science Postdoctoral Excellence Programme (BIOME PEP) of the Medical Faculty University Duisburg-Essen
ID : N/A
Organisme : Open Access Publication Fund of the University of Duisburg-Essen
ID : N/A

Références

N Engl J Med. 2004 Aug 19;351(8):781-91
pubmed: 15317891
Nat Rev Genet. 2019 Feb;20(2):71-88
pubmed: 30410101
Sci Transl Med. 2015 Nov 11;7(313):313ra182
pubmed: 26560360
Oncotarget. 2017 Jun 27;8(42):72054-72068
pubmed: 29069768
Oncotarget. 2016 Dec 20;7(51):85349-85364
pubmed: 27863403
Lancet Oncol. 2017 Jul;18(7):904-916
pubmed: 28576675
Cancers (Basel). 2019 Feb 18;11(2):
pubmed: 30781720
Lancet Oncol. 2014 Apr;15(4):406-14
pubmed: 24636208
Genes Chromosomes Cancer. 2019 Apr;58(4):219-232
pubmed: 30382599
Mol Cancer. 2018 Feb 26;17(1):67
pubmed: 29482551
Eur J Cancer. 2009 Jan;45(2):228-47
pubmed: 19097774
Clin Cancer Res. 2017 Jan 1;23(1):88-96
pubmed: 27334837
Breast Cancer Res. 2019 Dec 19;21(1):149
pubmed: 31856868
NPJ Genom Med. 2017 Nov 1;2:34
pubmed: 29263843
Science. 2017 Mar 17;355(6330):1152-1158
pubmed: 28302823
N Engl J Med. 2013 Mar 28;368(13):1199-209
pubmed: 23484797
BMC Genomics. 2008 Oct 16;9:488
pubmed: 18925949
Breast Cancer Res Treat. 2014 Jul;146(1):163-74
pubmed: 24838941
Nat Rev Cancer. 2011 Jun;11(6):426-37
pubmed: 21562580
Breast Cancer Res. 2014 Aug 09;16(4):421
pubmed: 25107527
JCI Insight. 2019 Mar 7;4(5):
pubmed: 30702443
JAMA Oncol. 2019 Feb 1;5(2):e184475
pubmed: 30543347
Nat Genet. 2013 Dec;45(12):1446-51
pubmed: 24185510
Cancer Discov. 2014 Jun;4(6):650-61
pubmed: 24801577
J Clin Oncol. 2016 Sep 1;34(25):2961-8
pubmed: 27269946
Cell Mol Life Sci. 2020 Feb;77(3):497-509
pubmed: 31254045
Clin Cancer Res. 2016 Dec 15;22(24):6010-6020
pubmed: 27281561
Oncotarget. 2016 May 3;7(18):26107-19
pubmed: 27034166
Semin Cancer Biol. 2017 Jun;44:106-116
pubmed: 28442298
Neoplasma. 2017;64(4):611-618
pubmed: 28485169
Oncol Res Treat. 2016;39(3):112-6
pubmed: 27031542
Breast Cancer Res. 2019 Dec 4;21(1):137
pubmed: 31801599
JAMA Oncol. 2016 Oct 1;2(10):1310-1315
pubmed: 27532364
Mol Oncol. 2015 Apr;9(4):749-57
pubmed: 25539732
Nat Commun. 2018 Apr 27;9(1):1691
pubmed: 29703982
Clin Cancer Res. 2018 Feb 1;24(3):560-568
pubmed: 29180605
Clin Cancer Res. 2018 Mar 15;24(6):1500-1510
pubmed: 29284708
Clin Cancer Res. 2016 Mar 1;22(5):1103-10
pubmed: 26446944
Cancer Res. 2018 Feb 15;78(4):1110-1122
pubmed: 29233927
Nat Rev Cancer. 2017 Apr;17(4):223-238
pubmed: 28233803
Clin Cancer Res. 2017 Oct 15;23(20):6086-6093
pubmed: 28679775
Oncotarget. 2016 May 3;7(18):26724-38
pubmed: 27049831
Oncotarget. 2017 Jun 14;8(32):52142-52155
pubmed: 28881720
Mol Oncol. 2019 Dec;13(12):2515-2530
pubmed: 31254443
Clin Chem. 2018 Jul;64(7):1054-1062
pubmed: 29769179
Mol Oncol. 2018 Jan;12(1):48-57
pubmed: 29063679
Nat Methods. 2015 Jun;12(6):519-22
pubmed: 25915121

Auteurs

Corinna Keup (C)

Department of Gynecology and Obstetrics, University Hospital of Essen, 45122 Essen, Germany.

Markus Storbeck (M)

QIAGEN, 40724 Hilden, Germany.

Siegfried Hauch (S)

QIAGEN, 40724 Hilden, Germany.

Peter Hahn (P)

QIAGEN, 40724 Hilden, Germany.

Markus Sprenger-Haussels (M)

QIAGEN, 40724 Hilden, Germany.

Oliver Hoffmann (O)

Department of Gynecology and Obstetrics, University Hospital of Essen, 45122 Essen, Germany.

Rainer Kimmig (R)

Department of Gynecology and Obstetrics, University Hospital of Essen, 45122 Essen, Germany.

Sabine Kasimir-Bauer (S)

Department of Gynecology and Obstetrics, University Hospital of Essen, 45122 Essen, Germany.

Classifications MeSH