Draft genome sequences of Hirudo medicinalis and salivary transcriptome of three closely related medicinal leeches.

Animals Anticoagulants / metabolism Gene Expression Profiling Gene Expression Regulation Genome Hirudo medicinalis / genetics Leeches / classification Proteomics Saliva / metabolism Salivary Proteins and Peptides / genetics
Anticoagulants Genome Haematophagy Leech H. medicinalis Systems biology

Journal

BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258

Informations de publication

Date de publication:
29 Apr 2020
Historique:
received: 03 10 2019
accepted: 21 04 2020
entrez: 1 5 2020
pubmed: 1 5 2020
medline: 6 1 2021
Statut: epublish

Résumé

Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis. Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches.

Sections du résumé

BACKGROUND BACKGROUND
Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today.
RESULTS RESULTS
We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, Hirudo orientalis, and Hirudo verbana. Differential expression analysis verified by proteomics identified salivary cell-specific gene expression, many of which encode previously unknown salivary components. However, the genes encoding known anticoagulants have been found to be expressed not only in salivary cells. The function-related analysis of the unique salivary cell genes enabled an update of the concept of interactions between salivary proteins and components of haemostasis.
CONCLUSIONS CONCLUSIONS
Here we report a genome draft of Hirudo medicinalis and describe identification of novel salivary proteins and new homologs of genes encoding known anticoagulants in transcriptomes of three medicinal leech species. Our data provide new insights in genetics of blood-feeding lifestyle in leeches.

Identifiants

DOI: 10.1186/s12864-020-6748-0 PMID: 32349672 PMC: PMC7191736
pubmed: 32349672
doi: 10.1186/s12864-020-6748-0
pii: 10.1186/s12864-020-6748-0
pmc: PMC7191736
doi:

Substances chimiques

Anticoagulants 0
Salivary Proteins and Peptides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

331

Subventions

Organisme : Russian Science Foundation
ID : 17-75-20099
Organisme : Russian Science Foundation
ID : 14-14-00696

Commentaires et corrections

Type : ErratumIn

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Auteurs

Vladislav V Babenko (VV)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia. daniorerio34@gmail.com.
ORCID: 0000-0003-2242-2127

Oleg V Podgorny (OV)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Koltzov Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov str, Moscow, 119334, Russia.

Valentin A Manuvera (VA)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.

Artem S Kasianov (AS)

Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.
Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina str, Moscow, 119991, Russia.

Alexander I Manolov (AI)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Ekaterina N Grafskaia (EN)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.

Dmitriy A Shirokov (DA)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Alexey S Kurdyumov (AS)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Dmitriy V Vinogradov (DV)

A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 19 Bol'shoi Karetnyi per, Moscow, 127051, Russia.
Skolkovo Institute of Science and Technology, 3 Nobelya Ulitsa str, Moscow, 121205, Russia.

Anastasia S Nikitina (AS)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.

Sergey I Kovalchuk (SI)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str, Moscow, 117997, Russia.

Nickolay A Anikanov (NA)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya str, Moscow, 117997, Russia.

Ivan O Butenko (IO)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Olga V Pobeguts (OV)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Daria S Matyushkina (DS)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Daria V Rakitina (DV)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Elena S Kostryukova (ES)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.

Victor G Zgoda (VG)

V.N. Orekhovich Research Institute of Biomedical Chemistry, Russian Academy of Medical Sciences, 10 Pogodinskaja str, Moscow, 119832, Russia.

Isolda P Baskova (IP)

Faculty of Biology, Lomonosov Moscow State University, 1-12 Leninskie Gory, Moscow, 119991, Russia.

Vladimir M Trukhan (VM)

I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University), Trubetskaya str., 8-2, Moscow, 119991, Russia.

Mikhail S Gelfand (MS)

A.A. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 19 Bol'shoi Karetnyi per, Moscow, 127051, Russia.
Skolkovo Institute of Science and Technology, 3 Nobelya Ulitsa str, Moscow, 121205, Russia.
Faculty of Computer Science, National Research University Higher School of Economics, 20 Myasnitskaya str, Moscow, 101000, Russia.
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 1-73 Leninskie Gory, Moscow, 119991, Russia.

Vadim M Govorun (VM)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.

Helgi B Schiöth (HB)

I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovskiy University), Trubetskaya str., 8-2, Moscow, 119991, Russia.
Functional Pharmacology, Department of Neuroscience, Uppsala University, Husargatan 3, Uppsala, 75124, Sweden.

Vassili N Lazarev (VN)

Federal Research and Clinical Centre of Physical-Chemical Medicine of Federal Medical Biological Agency, 1a Malaya Pirogovskaya Str, Moscow, 119435, Russia.
Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region, 141700, Russia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Agents hématologiques › Anticoagulants › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de profil d'expression de gènes › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Annelida › Sangsues › Hirudo medicinalis › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Annelida › Sangsues › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Liquides et sécrétions biologiques › Sécrétions corporelles › Salive › Draft genome sequences of Hirudo medicinalis...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides salivaires › Draft genome sequences of Hirudo medicinalis...