Metabolic regulation of the lysosomal cofactor bis(monoacylglycero)phosphate in mice.

adipose tissue body temperature insulin lipid metabolism liver lysosome nutritional state pancreas phospholipids

Journal

Journal of lipid research
ISSN: 1539-7262
Titre abrégé: J Lipid Res
Pays: United States
ID NLM: 0376606

Informations de publication

Date de publication:
07 2020
Historique:
received: 04 11 2019
revised: 23 04 2020
pubmed: 1 5 2020
medline: 7 8 2021
entrez: 1 5 2020
Statut: ppublish

Résumé

Bis(monoacylglycero)phosphate (BMP), also known as lysobisphosphatidic acid, is a phospholipid that promotes lipid sorting in late endosomes/lysosomes by activating lipid hydrolases and lipid transfer proteins. Changes in the cellular BMP content therefore reflect an altered metabolic activity of the endolysosomal system. Surprisingly, little is known about the physiological regulation of BMP. In this study, we investigated the effects of nutritional and metabolic factors on BMP profiles of whole tissues and parenchymal and nonparenchymal cells. Tissue samples were obtained from fed, fasted, 2 h refed, and insulin-treated mice, as well as from mice housed at 5°C, 22°C, or 30°C. These tissues exhibited distinct BMP profiles that were regulated by the nutritional state in a tissue-specific manner. Insulin treatment was not sufficient to mimic refeeding-induced changes in tissue BMP levels, indicating that BMP metabolism is regulated by other hormonal or nutritional factors. Tissue fractionation experiments revealed that fasting drastically elevates BMP levels in hepatocytes and pancreatic cells. Furthermore, we observed that the BMP content in brown adipose tissue strongly depends on housing temperatures. In conclusion, our observations suggest that BMP concentrations adapt to the metabolic state in a tissue- and cell-type-specific manner in mice. Drastic changes observed in hepatocytes, pancreatic cells, and brown adipocytes suggest that BMP plays a role in the functional adaption to nutrient starvation and ambient temperature.

Identifiants

pubmed: 32350080
pii: S0022-2275(20)43578-3
doi: 10.1194/jlr.RA119000516
pmc: PMC7328040
pii:
doi:

Substances chimiques

Lysophospholipids 0
Monoglycerides 0
bis(monoacylglyceryl)phosphate 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

995-1003

Subventions

Organisme : Austrian Science Fund FWF
ID : P 28882
Pays : Austria

Informations de copyright

Copyright © 2020 Grabner et al. Published by The American Society for Biochemistry and Molecular Biology, Inc.

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Auteurs

Gernot F Grabner (GF)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Nermeen Fawzy (N)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Renate Schreiber (R)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Lisa M Pusch (LM)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Dominik Bulfon (D)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Harald Koefeler (H)

Otto Loewi Research Center, and Center for Medical Research, Medical University of Graz, Graz, Austria; BioTechMed-Graz, Graz, Austria.

Thomas O Eichmann (TO)

Institute of Molecular Biosciences, University of Graz, Graz, Austria; Center for Explorative Lipidomics, BioTechMed-Graz, Graz, Austria.

Achim Lass (A)

Institute of Molecular Biosciences, University of Graz, Graz, Austria; BioTechMed-Graz, Graz, Austria.

Martina Schweiger (M)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Gunther Marsche (G)

Division of Pharmacology, Medical University of Graz, Graz, Austria; BioTechMed-Graz, Graz, Austria.

Gabriele Schoiswohl (G)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Ulrike Taschler (U)

Institute of Molecular Biosciences, University of Graz, Graz, Austria.

Robert Zimmermann (R)

Institute of Molecular Biosciences, University of Graz, Graz, Austria; BioTechMed-Graz, Graz, Austria. Electronic address: mailto:robert.zimmermann@uni-graz.at.

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Classifications MeSH