Astrovirus infects actively secreting goblet cells and alters the gut mucus barrier.
Animals
Astroviridae
/ physiology
Astroviridae Infections
/ pathology
Epithelial Cells
/ pathology
Escherichia coli
/ physiology
Female
Gastrointestinal Tract
/ microbiology
Goblet Cells
/ virology
Male
Mice, Inbred C57BL
Mucus
/ microbiology
Transcriptome
/ genetics
Virus Replication
/ physiology
Virus Shedding
/ physiology
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 04 2020
29 04 2020
Historique:
received:
24
10
2019
accepted:
08
04
2020
entrez:
1
5
2020
pubmed:
1
5
2020
medline:
30
7
2020
Statut:
epublish
Résumé
Astroviruses are a global cause of pediatric diarrhea, but they are largely understudied, and it is unclear how and where they replicate in the gut. Using an in vivo model, here we report that murine astrovirus preferentially infects actively secreting small intestinal goblet cells, specialized epithelial cells that maintain the mucus barrier. Consequently, virus infection alters mucus production, leading to an increase in mucus-associated bacteria and resistance to enteropathogenic E. coli colonization. These studies establish the main target cell type and region of the gut for productive murine astrovirus infection. They further define a mechanism by which an enteric virus can regulate the mucus barrier, induce functional changes to commensal microbial communities, and alter host susceptibility to pathogenic bacteria.
Identifiants
pubmed: 32350281
doi: 10.1038/s41467-020-15999-y
pii: 10.1038/s41467-020-15999-y
pmc: PMC7190700
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2097Subventions
Organisme : NIAID NIH HHS
ID : R21 AI135254
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI106700
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA021765
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI136514
Pays : United States
Organisme : NIAID NIH HHS
ID : R03 AI126101
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI121832
Pays : United States
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