Comparative mitochondrial proteomics of Leishmania tropica clinical isolates resistant and sensitive to meglumine antimoniate.
Animals
Antiprotozoal Agents
/ pharmacology
Cell Line
Chromatography, Liquid
Drug Resistance
Leishmania tropica
/ drug effects
Meglumine Antimoniate
/ pharmacology
Mice
Mitochondria
/ drug effects
Mitochondrial Proteins
/ metabolism
Parasitic Sensitivity Tests
Proteome
Proteomics
Tandem Mass Spectrometry
LC-MS/MS
Leishmania
Meglumine antimoniate
Mitochondrial proteomics
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
11
05
2019
accepted:
18
03
2020
pubmed:
1
5
2020
medline:
11
7
2020
entrez:
1
5
2020
Statut:
ppublish
Résumé
Antimony is an important drug for the treatment of Leishmania parasite infections. In several countries, the emergence of drug-resistant Leishmania species has reduced the effectiveness of this drug. The mechanism of clinical drug resistance is unclear. The aim of this work was to identify mitochondrial proteome alterations associated with resistance against antimonial. A combination of cell fractionation, liquid chromatography-tandem mass spectrometry (LC-MS/MS), and Label-Free Quantification was used to characterize the mitochondrial protein composition of Leishmania tropica field isolates resistant and sensitive to meglumine antimoniate. LC-MS/MS analysis resulted in the identification of about 1200 proteins of the Leishmania tropica mitochondrial proteome. Various criteria were used to allocate about 40% proteins to mitochondrial proteome. Comparative quantitative proteomic analysis of the sensitive and the resistant strains showed proteins with differential abundance in resistance species are involved in TCA and aerobic respiration enzymes, stress proteins, lipid metabolism enzymes, and translation. These results showed that the mechanism of antimony resistance in Leishmania spp. field isolate may be associated with alteration in enzymes involved in mitochondrial pathways.
Identifiants
pubmed: 32350589
doi: 10.1007/s00436-020-06671-x
pii: 10.1007/s00436-020-06671-x
doi:
Substances chimiques
Antiprotozoal Agents
0
Mitochondrial Proteins
0
Proteome
0
Meglumine Antimoniate
75G4TW236W
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1857-1871Subventions
Organisme : Iran University of Medical Sciences (IUMS), Tehran, Iran
ID : Grant No.28625
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