Avian uterine fluid proteome: Exosomes and biological processes potentially involved in sperm survival.
Animals
Annexin A4
/ metabolism
Biological Phenomena
Biomarkers
/ metabolism
Body Fluids
/ chemistry
Chickens
/ physiology
Exosomes
/ chemistry
Female
Male
Protein Deglycase DJ-1
/ metabolism
Proteome
Proteomics
Semen
/ chemistry
Spermatozoa
/ metabolism
Uterus
/ metabolism
Valosin Containing Protein
/ metabolism
avian
exosomes
proteome
sperm
uterine fluid
Journal
Molecular reproduction and development
ISSN: 1098-2795
Titre abrégé: Mol Reprod Dev
Pays: United States
ID NLM: 8903333
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
30
09
2019
accepted:
31
01
2020
entrez:
1
5
2020
pubmed:
1
5
2020
medline:
21
5
2021
Statut:
ppublish
Résumé
Uterine fluid is an aqueous milieu to which sperm are exposed during their storage and ascent. In this study, a bottom-up proteomic strategy and bioinformatic analysis of hen uterine fluid was performed to improve the understanding of this fluid and its potential role in sperm survival mechanisms. The proteomic data were submitted to ProteomeXchange. Among the 913 proteins identified, 160 are known to be secreted and 640 are referenced in exosomes databases. We isolated exosomes from the avian uterine fluid, analyzed them using electron microscopy, and targeted several exosomes markers (ANXA1/2/4/5, VCP, HSP90A, HSPA8, PARK7, and MDH1) using immunoblotting. Electron microscopy and immunohistochemistry were also used to analyze uterovaginal junctions for the exosomal proteins ANXA4, VCP, and PARK7. Exosomes were observed both at the surface epithelium and inside sperm storage tubules. Our data were compared with two previously published studies on proteomic of hen uterine fluid, and with one study describing the proteomic content of rooster seminal plasma and sperm. In conclusion, we demonstrated for the first time that avian uterine fluid contains exosomes. These may play a key role in preserving sperm functions within the female genital tract. Their presence in the sperm storage tubules may represent an important mechanism regarding interaction between the female genital tract and sperm.
Substances chimiques
Annexin A4
0
Biomarkers
0
Proteome
0
Protein Deglycase DJ-1
EC 3.1.2.-
Valosin Containing Protein
EC 3.6.4.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
454-470Informations de copyright
© 2020 Wiley Periodicals, Inc.
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