Identification and Analysis of Components in Yizhi Granule and Cynomolgus Monkey Plasma after Oral Administration by UPLC/ESI-Q-TOF MS and Their Protective Effects on PC12 Cells.


Journal

Journal of analytical methods in chemistry
ISSN: 2090-8865
Titre abrégé: J Anal Methods Chem
Pays: Egypt
ID NLM: 101575377

Informations de publication

Date de publication:
2020
Historique:
received: 17 12 2019
accepted: 28 01 2020
entrez: 1 5 2020
pubmed: 1 5 2020
medline: 1 5 2020
Statut: epublish

Résumé

Yizhi Granule (YZG) is a health food containing six traditional Chinese medicines (TCMs). It improves memory barriers in rat experiments. Here, we describe the first fast and sensitive ultraperformance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) method for analyzing YZG in plasma. We used this technique for studies in cynomolgus monkey plasma. By comparing retention time, MS, and MS/MS data of reference compounds, 70 compounds were detected in YZG. Of these, 63 were identified including 60 saponins, 2 flavones, and 1 methyl ester. There were 33 saponins, 1 flavone, and 1 methyl ester in the plasma. Next, to study the therapeutic properties of YZG, the neuroprotective effect of some of the absorbed components was evaluated using PC12 cell damage caused by the A

Identifiants

pubmed: 32351755
doi: 10.1155/2020/5165631
pmc: PMC7171651
doi:

Types de publication

Journal Article

Langues

eng

Pagination

5165631

Informations de copyright

Copyright © 2020 Erwei Hao et al.

Déclaration de conflit d'intérêts

The authors declare no conflicts of interest.

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Auteurs

Erwei Hao (E)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Postdoctoral Workstation, Guangxi Institue of Medicinal Plants, Nanning 530023, China.

Jianfeng Qin (J)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.

Wei Wei (W)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.

Jianhua Miao (J)

Postdoctoral Workstation, Guangxi Institue of Medicinal Plants, Nanning 530023, China.

Yan Xie (Y)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.

Xianglong Pan (X)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.

Hangxuan Wu (H)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.

Jinling Xie (J)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.

Xiaosu Fan (X)

Experimental Center of College of Agriculture, Guangxi University, Nanning 530005, China.

Zhengcai Du (Z)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Postdoctoral Workstation, Guangxi Institue of Medicinal Plants, Nanning 530023, China.

Xiaotao Hou (X)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Postdoctoral Workstation, Guangxi Institue of Medicinal Plants, Nanning 530023, China.

Jiagang Deng (J)

Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Collaborative Innovation Center for Research on Functional Ingredients of Agricultural Residues, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China.
Postdoctoral Workstation, Guangxi Institue of Medicinal Plants, Nanning 530023, China.

Classifications MeSH