Levitating Cells to Sort the Fit and the Fat.
cardiomyocytes
density
label-free sorting
lipid storage disease
magnetic levitation
Journal
Advanced biosystems
ISSN: 2366-7478
Titre abrégé: Adv Biosyst
Pays: Germany
ID NLM: 101711718
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
17
12
2019
revised:
12
03
2020
accepted:
30
03
2020
pubmed:
1
5
2020
medline:
12
8
2021
entrez:
1
5
2020
Statut:
ppublish
Résumé
Density is a core material property and varies between different cell types, mainly based on differences in their lipid content. Sorting based on density enables various biomedical applications such as multi-omics in precision medicine and regenerative repair in medicine. However, a significant challenge is sorting cells of the same type based on density differences. Here, a new method for real-time monitoring and sorting of single cells based on their inherent levitation profiles driven by their lipid content is reported. As a model system, human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) from a patient with neutral lipid storage disease (NLSD) due to loss of function of adipose triglyceride lipase (ATGL) resulting in abnormal lipid storage in cardiac muscle are used. This levitation-based strategy detects subpopulations within ATGL-deficient hiPSC-CMs with heterogenous lipid content, equilibrating at different levitation heights due to small density differences. In addition, sorting of these differentially levitating subpopulations are monitored in real time. Using this approach, sorted healthy and diseased hiPSC-CMs maintain viability and function. Pixel-tracking technologies show differences in contraction between NLSD and healthy hiPSC-CMs. Overall, this is a unique approach to separate diseased cell populations based on their intracellular lipid content that cannot be achieved using traditional flow cytometry techniques.
Identifiants
pubmed: 32352239
doi: 10.1002/adbi.201900300
doi:
Substances chimiques
Lipase
EC 3.1.1.3
PNPLA2 protein, human
EC 3.1.1.3
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1900300Subventions
Organisme : NLM NIH HHS
ID : DP1 LM012179
Pays : United States
Organisme : NIH HHS
ID : 5F32HL142205
Pays : United States
Organisme : NIH HHS
ID : R01 HL132875
Pays : United States
Organisme : NIH HHS
ID : UG3 TR002588
Pays : United States
Organisme : NIH Office of the Director
ID : LM012179-03
Pays : International
Organisme : NIH HHS
ID : R01 HL 113006
Pays : United States
Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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