Tunable self-cleaving ribozymes for modulating gene expression in eukaryotic systems.
Animals
Drosophila
/ genetics
Eukaryota
/ genetics
Eukaryotic Cells
/ metabolism
Gene Expression
/ genetics
Gene Expression Regulation
/ genetics
Genetic Engineering
/ methods
Nucleic Acid Conformation
Proof of Concept Study
RNA, Catalytic
/ genetics
RNA, Messenger
/ metabolism
Synthetic Biology
/ methods
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
05
02
2020
accepted:
05
04
2020
entrez:
1
5
2020
pubmed:
1
5
2020
medline:
21
7
2020
Statut:
epublish
Résumé
Advancements in the field of synthetic biology have been possible due to the development of genetic tools that are able to regulate gene expression. However, the current toolbox of gene regulatory tools for eukaryotic systems have been outpaced by those developed for simple, single-celled systems. Here, we engineered a set of gene regulatory tools by combining self-cleaving ribozymes with various upstream competing sequences that were designed to disrupt ribozyme self-cleavage. As a proof-of-concept, we were able to modulate GFP expression in mammalian cells, and then showed the feasibility of these tools in Drosophila embryos. For each system, the fold-reduction of gene expression was influenced by the location of the self-cleaving ribozyme/upstream competing sequence (i.e. 5' vs. 3' untranslated region) and the competing sequence used. Together, this work provides a set of genetic tools that can be used to tune gene expression across various eukaryotic systems.
Identifiants
pubmed: 32352996
doi: 10.1371/journal.pone.0232046
pii: PONE-D-20-03207
pmc: PMC7192461
doi:
Substances chimiques
RNA, Catalytic
0
RNA, Messenger
0
Banques de données
Dryad
['10.5061/dryad.3j9kd51dz']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0232046Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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