The contribution of plasma oxysterols in the challenging diagnostic work-up of infantile cholestasis.


Journal

Clinica chimica acta; international journal of clinical chemistry
ISSN: 1873-3492
Titre abrégé: Clin Chim Acta
Pays: Netherlands
ID NLM: 1302422

Informations de publication

Date de publication:
Aug 2020
Historique:
received: 05 03 2020
revised: 22 04 2020
accepted: 24 04 2020
pubmed: 1 5 2020
medline: 14 1 2021
entrez: 1 5 2020
Statut: ppublish

Résumé

Infantile cholestasis (IC) is defined as an impairment of bile production or flow occurring in the first months of life. The diagnostic approach in IC is challenging since the differential diagnosis is broad. We retrospectively evaluated 91 cholestatic infants referred to our department from 2014 to 2019. Patients with cholestasis underwent a complete IC diagnostic work-up including quantification of plasma oxysterols 7-ketocholesterol (7-KC) and cholestan-3β,5α,6β-triol (C-Triol). Oxysterols concentrations were mildly elevated in IC compared to control population. 7-KC and C-Triol plasma levels presented a linear relationship between them and with Spleen-Z score. Patients with NP-C showed the highest concentrations of both oxysterols compared with other etiologies of IC. Excluding NP-C patients, oxysterols concentrations were similar among all other etiological groups with no correlations found between them and the levels of cholesterol and bilirubin. ROC analysis identified AUCs of 1.0 for both oxysterols in predicting NP-C. Infants with IC should undergo a stepwise evaluation in which detailed clinical and deep analytical assessments are the main crossroads. Plasma oxysterols, a simple, reliable, and convenient diagnostic test should be included in the first steps of the diagnostic process in IC.

Sections du résumé

BACKGROUND BACKGROUND
Infantile cholestasis (IC) is defined as an impairment of bile production or flow occurring in the first months of life. The diagnostic approach in IC is challenging since the differential diagnosis is broad.
METHODS METHODS
We retrospectively evaluated 91 cholestatic infants referred to our department from 2014 to 2019. Patients with cholestasis underwent a complete IC diagnostic work-up including quantification of plasma oxysterols 7-ketocholesterol (7-KC) and cholestan-3β,5α,6β-triol (C-Triol).
RESULTS RESULTS
Oxysterols concentrations were mildly elevated in IC compared to control population. 7-KC and C-Triol plasma levels presented a linear relationship between them and with Spleen-Z score. Patients with NP-C showed the highest concentrations of both oxysterols compared with other etiologies of IC. Excluding NP-C patients, oxysterols concentrations were similar among all other etiological groups with no correlations found between them and the levels of cholesterol and bilirubin. ROC analysis identified AUCs of 1.0 for both oxysterols in predicting NP-C.
CONCLUSION CONCLUSIONS
Infants with IC should undergo a stepwise evaluation in which detailed clinical and deep analytical assessments are the main crossroads. Plasma oxysterols, a simple, reliable, and convenient diagnostic test should be included in the first steps of the diagnostic process in IC.

Identifiants

pubmed: 32353361
pii: S0009-8981(20)30182-0
doi: 10.1016/j.cca.2020.04.028
pii:
doi:

Substances chimiques

Oxysterols 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

181-186

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest None.

Auteurs

Andrea Pietrobattista (A)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy. Electronic address: andrea.pietrobattista@opbg.net.

Silvio Veraldi (S)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy; Sapienza University of Rome, Department of Pediatrics, Rome, Italy.

Manila Candusso (M)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Maria Sole Basso (MS)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Daniela Liccardo (D)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Claudia Della Corte (C)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Antonella Mosca (A)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Tommaso Alterio (T)

Division of Hepatology, Gastroenterology and Nutrition, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Elisa Sacchetti (E)

Division of Metabolism, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Giulio Catesini (G)

Division of Metabolism, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Federica Deodato (F)

Division of Metabolism, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Sara Boenzi (S)

Division of Metabolism, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

Carlo Dionisi-Vici (C)

Division of Metabolism, Bambino Gesù Children Hospital and Research Institute, Rome, Italy.

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Classifications MeSH