An LC-MS Assay to Measure Superoxide Radicals and Hydrogen Peroxide in the Blood System.
Plasmodium falciparum
human plasma
hydrogen peroxide species
liquid-chromatography
mass spectrometry
microvesicles
red blood cells
superoxide radicals
Journal
Metabolites
ISSN: 2218-1989
Titre abrégé: Metabolites
Pays: Switzerland
ID NLM: 101578790
Informations de publication
Date de publication:
28 Apr 2020
28 Apr 2020
Historique:
received:
13
03
2020
revised:
23
04
2020
accepted:
24
04
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
2
5
2020
Statut:
epublish
Résumé
Red blood cells are constantly exposed to reactive species under physiological or pathological conditions or during administration of xenobiotics. Regardless of the source, its accurate quantification is paramount in the area of theragnostics, which had been elusive up until now. Even if there are a lot of approaches to evaluate the oxidative stress, very sensitive methods are missing for the blood system. We therefore sought to apply a highly sensitive approach, by liquid chromatography coupled to mass spectrometry (UPLC-MS), for the quantification of reactive species such as superoxide radical and hydrogen peroxide using dihydroethidium (DHE) and coumarin boronic acid (CBA) probes respectively through the detection of 2-hydroxyethidium (2OH-E
Identifiants
pubmed: 32354089
pii: metabo10050175
doi: 10.3390/metabo10050175
pmc: PMC7280988
pii:
doi:
Types de publication
Journal Article
Langues
eng
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