A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids.
3D cell culture models
analytical device
flow-reactors
mass density
multiparametric characterization
spheroids
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
28 Apr 2020
28 Apr 2020
Historique:
received:
23
03
2020
revised:
23
04
2020
accepted:
28
04
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
2
5
2020
Statut:
epublish
Résumé
Gathering precise information on mass density, size and weight of cells or cell aggregates, is crucial for applications in many biomedical fields with a specific focus on cancer research. Although few technical solutions have been presented for single-cell analysis, literature does not cover this aspect for 3D models such as spheroids. Since the research interest on such samples is notably rising, here we describe a flow-apparatus, and the associated physical method and operative protocol for the accurate measurements of mass density, size and weight. The technique is based on the detection of the terminal velocity of a free-falling sample into a specifically conceived analysis flow-channel. Moreover, in order to demonstrate the accuracy and precision of the presented flow-device, analyses were initially carried out on standardized polystyrene beads. Finally, to display the application of the proposed system for biological samples, mass density, size and weight of live SW620 tumor spheroids were analyzed. The combined measurements of such parameters can represent a step toward a deeper understanding of 3D culture models.
Identifiants
pubmed: 32354148
pii: mi11050465
doi: 10.3390/mi11050465
pmc: PMC7281630
pii:
doi:
Types de publication
Journal Article
Langues
eng
Déclaration de conflit d'intérêts
Authors of Affiliation 1 are employees by CellDynamics isrl. The authors declare no conflict of interest. Daniele Gazzola, Simone Bonetti, Domenico Andrea Cristaldi, Azzurra Sargenti and Francesco Musmeci are the inventors of Patent No. 102020000006031.
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