Continuous Detection of Increasing Concentrations of Thrombin Employing a Label-Free Photonic Crystal Aptasensor.
aptasensor
label-free
optical biosensor
photonic bandgap
photonic crystal
thrombin
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
28 Apr 2020
28 Apr 2020
Historique:
received:
08
04
2020
revised:
23
04
2020
accepted:
27
04
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
2
5
2020
Statut:
epublish
Résumé
Thrombin generation is a complex and finely regulated pathway that provokes dynamical changes of thrombin concentration in blood when a vascular injury occurs. In order to characterize the initiation phase of such process, when thrombin concentration is in the nmol/L range, a label-free optical aptasensor is proposed here. This aptasensor combines a 1D photonic crystal structure consisting of a silicon corrugated waveguide with thrombin binding aptamers on its surface as bioreceptors. As a result, this aptasensor has been demonstrated to specifically detect thrombin concentrations ranging from 270 pmol/L to 27 nmol/L with an estimated detection limit of 33.5 pmol/L and a response time of ~2 min. Furthermore, it has also been demonstrated that this aptasensor is able to continuously respond to consecutive increasing concentrations of thrombin and to detect binding events as they occur. All these features make this aptasensor a good candidate to continuously study how thrombin concentration progressively increases during the initiation phase of the coagulation cascade.
Identifiants
pubmed: 32354154
pii: mi11050464
doi: 10.3390/mi11050464
pmc: PMC7281654
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Generalitat Valenciana
ID : PROMETEO - AVANTI/2019/123
Organisme : Universitat Politècnica de València
ID : PAID-01-17
Organisme : European Commission
ID : ERDF - Valencian Community 2014-2020
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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