Cultured macrophages transfer surplus cholesterol into adjacent cells in the absence of serum or high-density lipoproteins.
ATP Binding Cassette Transporter 1
/ metabolism
ATP-Binding Cassette Transporters
/ metabolism
Animals
Atherosclerosis
/ metabolism
Biological Transport
Cholesterol
/ metabolism
Foam Cells
/ metabolism
Lipid Metabolism
Lipoproteins, HDL
/ metabolism
Macrophages
/ metabolism
Macrophages, Peritoneal
/ metabolism
Mice
Mice, Inbred C57BL
Myocytes, Smooth Muscle
/ drug effects
Serum
/ metabolism
beta-Cyclodextrins
/ metabolism
cholesterol
macrophages
nanoSIMS imaging
smooth muscle cells
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
12 05 2020
12 05 2020
Historique:
pubmed:
2
5
2020
medline:
22
8
2020
entrez:
2
5
2020
Statut:
ppublish
Résumé
Cholesterol-laden macrophage foam cells are a hallmark of atherosclerosis. For that reason, cholesterol metabolism in macrophages has attracted considerable scrutiny, particularly the mechanisms by which macrophages unload surplus cholesterol (a process referred to as "cholesterol efflux"). Many studies of cholesterol efflux in macrophages have focused on the role of ABC transporters in moving cholesterol onto high-density lipoproteins (HDLs), but other mechanisms for cholesterol efflux likely exist. We hypothesized that macrophages have the capacity to unload cholesterol directly onto adjacent cells. To test this hypothesis, we used methyl-β-cyclodextrin (MβCD) to load mouse peritoneal macrophages with [
Identifiants
pubmed: 32354992
pii: 1922879117
doi: 10.1073/pnas.1922879117
pmc: PMC7229689
doi:
Substances chimiques
ATP Binding Cassette Transporter 1
0
ATP-Binding Cassette Transporters
0
Lipoproteins, HDL
0
beta-Cyclodextrins
0
methyl-beta-cyclodextrin
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10476-10483Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL087228
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL146358
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL125335
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL090553
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL139725
Pays : United States
Commentaires et corrections
Type : CommentIn
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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