Expansion microscopy of
Animals
Animals, Genetically Modified
Caenorhabditis elegans
/ chemistry
Caenorhabditis elegans Proteins
/ analysis
Connexins
/ analysis
DNA
/ analysis
Fluorescent Antibody Technique
Luminescent Proteins
/ analysis
Microscopy, Fluorescence
Nanotechnology
Neurons
/ chemistry
RNA
/ analysis
Synapses
/ chemistry
Tissue Fixation
C. elegans
developmental biology
expansion microscopy
gene expression
immunohistochemistry
in situ hybridization
neuroscience
super-resolution imaging
synapses
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
01 05 2020
01 05 2020
Historique:
received:
20
02
2019
accepted:
30
03
2020
entrez:
2
5
2020
pubmed:
2
5
2020
medline:
16
3
2021
Statut:
epublish
Résumé
We recently developed expansion microscopy (ExM), which achieves nanoscale-precise imaging of specimens at ~70 nm resolution (with ~4.5x linear expansion) by isotropic swelling of chemically processed, hydrogel-embedded tissue. ExM of
Identifiants
pubmed: 32356725
doi: 10.7554/eLife.46249
pii: 46249
pmc: PMC7195193
doi:
pii:
Substances chimiques
Caenorhabditis elegans Proteins
0
Connexins
0
Luminescent Proteins
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Science Foundation
ID : 1734870
Pays : International
Organisme : NIBIB NIH HHS
ID : T32 EB001680
Pays : United States
Organisme : U.S. Army Research Laboratory
ID : W911NF1510548
Pays : International
Organisme : NIBIB NIH HHS
ID : R01 EB024261
Pays : United States
Organisme : Intelligence Advanced Research Projects Activity
ID : D16PC00008
Pays : International
Organisme : NIMH NIH HHS
ID : R01 MH110932
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA029639
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH114031
Pays : United States
Organisme : NHGRI NIH HHS
ID : RM1 HG008525
Pays : United States
Organisme : NINDS NIH HHS
ID : R35 NS105092
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH103910
Pays : United States
Organisme : Army Research Office
ID : W911NF1510548
Pays : International
Organisme : NIH HHS
ID : 1R01MH103910
Pays : United States
Informations de copyright
© 2020, Yu et al.
Déclaration de conflit d'intérêts
CY, AW, AS, FC Is a co-inventor on patents related to expansion microscopy: WO2015127183A2, US20170089811A1, US20160305856A1, US20170067096A1, US20160304952A1, WO2017147435A1, US20190256633A1, and WO2020013833A1, NB, AB, SA, CZ, MG, GH No competing interests declared, OH Reviewing editor, eLife, EB Is a co-inventor on patents related to expansion microscopy: WO2015127183A2, US20170089811A1, US20160305856A1, US20170067096A1, US20160304952A1, WO2017147435A1, US20190256633A1, and WO2020013833A1 and a co-founder of a company seeking to commercialize medical applications of expansion microscopy.
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