Evaluation of seven commercial African swine fever virus detection kits and three Taq polymerases on 300 well-characterized field samples.
Actins
/ genetics
African Swine Fever
/ diagnosis
African Swine Fever Virus
/ genetics
Animals
Belgium
/ epidemiology
Capsid Proteins
/ genetics
DNA, Viral
/ genetics
Molecular Diagnostic Techniques
/ veterinary
Reagent Kits, Diagnostic
/ veterinary
Real-Time Polymerase Chain Reaction
/ veterinary
Sensitivity and Specificity
Sus scrofa
Swine
Taq Polymerase
/ metabolism
African swine fever
Real-time PCR
Wild boar
Journal
Journal of virological methods
ISSN: 1879-0984
Titre abrégé: J Virol Methods
Pays: Netherlands
ID NLM: 8005839
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
25
02
2020
revised:
07
04
2020
accepted:
10
04
2020
pubmed:
4
5
2020
medline:
25
5
2021
entrez:
4
5
2020
Statut:
ppublish
Résumé
African swine fever virus (ASFV) is a complex double stranded DNA virus, responsible for a highly infectious and fatal disease in pigs and boars and for important deterioration of animal welfare. Over the last decade, the disease spread to several European and Asian countries causing unprecedented dramatic economic losses in pig industry. In the absence of a vaccine, affected countries rely on trustful diagnostic tests and adapted testing policies to set up control programs to fight against the disease. In this study, we evaluated the sensitivity and specificity of seven commercially available ASFV real-time PCR detection kits and three Taq polymerases on 300 well-characterized wild boar samples collected in Belgium during the 2018-2019 outbreak. This study confirms that all commercial kits and two Taq polymerases are suitable for ASFV detection in diagnostic laboratories. Furthermore, the use of endogenous controls is emphasized when testing field samples harvested on carcasses in an advanced stage of decomposition, in order to avoid false negative results.
Identifiants
pubmed: 32360149
pii: S0166-0934(20)30126-9
doi: 10.1016/j.jviromet.2020.113874
pii:
doi:
Substances chimiques
Actins
0
Capsid Proteins
0
DNA, Viral
0
Reagent Kits, Diagnostic
0
capsid protein p72, African swine fever virus
0
Taq Polymerase
EC 2.7.7.-
Types de publication
Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113874Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflicts of interest.