Control of adhesive ligand density for modulation of nucleus pulposus cell phenotype.
Intervertebral disc
Laminin-mimetic peptides
Ligand presentation
Nucleus pulposus
Substrate stiffness
Journal
Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
18
12
2019
revised:
29
03
2020
accepted:
16
04
2020
pubmed:
4
5
2020
medline:
15
5
2021
entrez:
4
5
2020
Statut:
ppublish
Résumé
Cells of the nucleus pulposus have been observed to undergo a shift from their notochordal-like juvenile phenotype to a more fibroblast-like state with age and maturation. It has been demonstrated that culture of degenerative adult human nucleus pulposus cells upon soft (<1 kPa) full length laminin-containing hydrogel substrates promotes increased levels of a panel of markers associated with the juvenile nucleus pulposus cell phenotype. In the current work, we observed an ability to use soft polymeric substrates functionalized with short laminin-mimetic peptide sequences to recapitulate the behaviors elicited by soft, full-length laminin containing materials. Furthermore, our work suggests an ability to mimic features of soft systems through control of peptide density upon stiffer substrates. Specifically, results suggest that stiffer polymer-peptide hydrogel substrates can be used to promote the expression of a more juvenile-like phenotype for cells of the nucleus pulposus by reducing adhesive ligand presentation. Here we show how polymer stiffness combined with adhesive ligand presentation can be controlled to be supportive of nucleus pulposus cell phenotype and biosynthesis.
Identifiants
pubmed: 32361392
pii: S0142-9612(20)30303-3
doi: 10.1016/j.biomaterials.2020.120057
pmc: PMC7608136
mid: NIHMS1589453
pii:
doi:
Substances chimiques
Adhesives
0
Laminin
0
Ligands
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
120057Subventions
Organisme : NIAMS NIH HHS
ID : F32 AR070579
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR069588
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM128764
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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