Expression of corticosteroid hormone receptors, prereceptors, and molecular chaperones in hypothalamic-pituitary-adrenal axis and adipose tissue after the administration of growth promoters in veal calves.
Adipose Tissue
/ metabolism
Adrenal Glands
/ drug effects
Animals
Cattle
/ physiology
Dexamethasone
/ administration & dosage
Estradiol
/ administration & dosage
Gene Expression Regulation
/ drug effects
Glucocorticoids
/ pharmacology
Hypothalamo-Hypophyseal System
/ metabolism
Hypothalamus
/ drug effects
Male
Molecular Chaperones
/ metabolism
Pituitary Gland
/ drug effects
Pituitary-Adrenal System
/ metabolism
Receptors, Steroid
/ metabolism
Adipose tissue
Calves
FKBP5
Glucocorticoid-responsive genes
Growth promoters
Journal
Domestic animal endocrinology
ISSN: 1879-0054
Titre abrégé: Domest Anim Endocrinol
Pays: United States
ID NLM: 8505191
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
15
05
2019
revised:
14
02
2020
accepted:
01
03
2020
pubmed:
4
5
2020
medline:
24
7
2021
entrez:
4
5
2020
Statut:
ppublish
Résumé
The action of glucocorticoids on target tissues is regulated by the glucocorticoid and mineralocorticoid receptors (codified by the NR3C1 and NR3C2 gene, respectively). Moreover, the prereceptor system, represented by the hydroxysteroid 11-beta dehydrogenases (HSD11Bs), catalyzes the interconversion from active glucocorticoids into inactive compounds. This study aimed to determine whether the expression of the prereceptor system, the corticosteroid receptors, and the molecules regulating their intracellular trafficking (FKBP prolyl isomerase 4 and FKBP prolyl isomerase 5) could be regulated in the hypothalamic-pituitary-adrenal axis and in different type of adipose tissue of calves by the administration of dexamethasone in combination with estradiol or prednisolone. Research about the glucocorticoid effects on bovine target tissues may allow development of new diagnostic methods that use potential molecular biomarkers of glucocorticoid treatment. The administration of dexamethasone in combination with estradiol increased the gene expression of HSD11B1 (P < 0.01), HSD11B2 (P < 0.05), NR3C1 (P < 0.01), and NR3C2 (P < 0.01) in the adrenal glands; NR3C2 in the intramuscular adipose tissue (P < 0.01), and HSD11B1 in the subcutaneous adipose tissue (P < 0.01). Prednisolone administration increased the gene expression of HSD11B1 (P < 0.01), NR3C1 (P < 0.05), and NR3C2 (P < 0.05) in the adrenal glands and HSD11B1 (P < 0.01) in the subcutaneous adipose tissue. Interestingly, most of the examined tissues/organs showed a significant variation of FKBP5 gene expression after the administration of dexamethasone in combination with estradiol. So, these changes suggest that the FKBP5 gene expression could be a possible biomarker of the illegal dexamethasone administration in calves.
Identifiants
pubmed: 32361423
pii: S0739-7240(20)30040-0
doi: 10.1016/j.domaniend.2020.106473
pii:
doi:
Substances chimiques
Glucocorticoids
0
Molecular Chaperones
0
Receptors, Steroid
0
estradiol 3-benzoate
1S4CJB5ZGN
Estradiol
4TI98Z838E
Dexamethasone
7S5I7G3JQL
Types de publication
Journal Article
Randomized Controlled Trial, Veterinary
Langues
eng
Sous-ensembles de citation
IM
Pagination
106473Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.