ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils.
Animals
Cell Differentiation
/ drug effects
Cytokines
/ immunology
Dimethyl Sulfoxide
/ pharmacology
Endocytosis
/ drug effects
HL-60 Cells
Humans
Inflammation
/ immunology
Mice, Inbred BALB C
Mice, Inbred C57BL
Mitogen-Activated Protein Kinases
/ antagonists & inhibitors
Neutrophils
/ drug effects
Particulate Matter
/ pharmacology
Protein Kinase Inhibitors
/ pharmacology
Reactive Oxygen Species
/ metabolism
Tretinoin
/ pharmacology
ATRA
HL-60
MAPK
Neutrophil
Particulate matter
Journal
International immunopharmacology
ISSN: 1878-1705
Titre abrégé: Int Immunopharmacol
Pays: Netherlands
ID NLM: 100965259
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
22
01
2020
revised:
03
04
2020
accepted:
12
04
2020
pubmed:
4
5
2020
medline:
11
3
2021
entrez:
4
5
2020
Statut:
ppublish
Résumé
Reports show that particulate matter (PM) is related to respiratory and cardiovascular diseases. We previously reported the biological effects of PM in vivo and the endocytosis of PM by primary neutrophils from mice. Cell lines can be used to elucidate the mechanism underlying immune responses in detail; however, information is limited regarding the functions of neutrophils after PM exposure. Here, we investigated the immune response of primary neutrophils and dimethyl sulfoxide (DMSO)- and all-trans retinoic acid (ATRA)-differentiated HL-60 (neutrophil-like) cells to PM. We showed that endocytosis by ATRA-HL cells was enhanced compared to that by DMSO-HL cells and that endocytosis in both cells was inhibited by dynamin inhibitors. A MEK inhibitor, but not p38 or JNK inhibitors, inhibited endocytosis. The MEK inhibitor also inhibited the differentiation of ATRA-HL cells to neutrophils. We identified that endocytosis of PM by neutrophils activated the MAPK ERK and p38 pathways. DMSO-HL and ATRA-HL cells both produced TNF-α and IL-8 after lipopolysaccharide (LPS) or PM treatment, whereas non-differentiated HL-60 cells did not. MCP-1 production was enhanced in DMSO-HL cells after LPS or PM treatment, whereas it was high in ATRA-HL cells. Reactive oxygen species (ROS) production was enhanced after PM treatment to DMSO-HL cells. Further, extracellular extracts promoted endocytosis. The MEK inhibitor also reduced the production of TNF-α, IL-8, and MCP-1. Taken together, ERK activation is key for both differentiation and endocytosis, and DMSO-HL cells at day 6 can serve as a model of inflammatory neutrophils, such as bronchus neutrophils, and a good tool to analyze the molecular events involved in immune responses to PM.
Identifiants
pubmed: 32361568
pii: S1567-5769(20)30213-7
doi: 10.1016/j.intimp.2020.106510
pii:
doi:
Substances chimiques
Cytokines
0
Particulate Matter
0
Protein Kinase Inhibitors
0
Reactive Oxygen Species
0
Tretinoin
5688UTC01R
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Dimethyl Sulfoxide
YOW8V9698H
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106510Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest No authors have any conflict of interest regarding the work.