Evaluation of xanthine oxidase inhibitory activity of flavonoids by an online capillary electrophoresis-based immobilized enzyme microreactor.
Capillary electrophoresis
Immobilized enzyme microreactor
Inhibitory activity
Xanthine oxidase
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
09
02
2020
revised:
25
04
2020
accepted:
29
04
2020
pubmed:
5
5
2020
medline:
23
3
2021
entrez:
5
5
2020
Statut:
ppublish
Résumé
Xanthine oxidase (XOD) is a key enzyme in the human body to produce uric acid, and its inhibitor can be used for the treatment of hyperuricemia and gout. In this study, an online CE-based XOD immobilized enzyme microreactor (IMER) was developed for the enzyme kinetics assays and inhibitor screening. After 30 consecutive runs, the XOD activity remained about 95.6% of the initial immobilized activity. The Michaelis-Menten constant (K
Identifiants
pubmed: 32363581
doi: 10.1002/elps.202000083
doi:
Substances chimiques
Enzymes, Immobilized
0
Flavonoids
0
Xanthine Oxidase
EC 1.17.3.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1326-1332Subventions
Organisme : Natural Science Foundation of Chongqing, China
ID : cstc2019jcyj-msxmX0074
Pays : International
Informations de copyright
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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