Significance of CD10 protein expression in the diagnostics of follicular lymphoma: A comparison of conventional immunohistochemistry with flow cytometry supported by the establishment of BCL2 and BCL6 rearrangements.
CD10 expression
fine-needle aspiration biopsy
flow cytometry
follicular lymphoma
immunohistochemistry
Journal
International journal of laboratory hematology
ISSN: 1751-553X
Titre abrégé: Int J Lab Hematol
Pays: England
ID NLM: 101300213
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
15
11
2019
revised:
12
01
2020
accepted:
09
04
2020
pubmed:
5
5
2020
medline:
6
1
2021
entrez:
5
5
2020
Statut:
ppublish
Résumé
Histopathological examination and immunohistochemistry (IHC) with a crucial role of CD10 expression remain a standard diagnostic tool in follicular lymphoma (FL). The results of IHC CD10 detection with different primary antibodies are not fully reproducible, but some reports show that flow cytometry (FCM) can be a reliable method of CD10 identification. The aim of the study was to compare results of CD10 expression in FL by IHC and FCM including immunophenotypic features in the context of the BCL2 and BCL6 alterations. Out of 76 histopathologically diagnosed FL, a group of 25 cases had simultaneously FCM. Immunohistochemically 77.6% of cases were CD10-positive with comparable and reproducible results to FCM. Differences between the FCM expression of CD5/CD10/CD11c/CD25/CD43 and BCL2 overexpression (BCL2(+) Immunohistochemistry staining is a comparable method to FCM assessment in the evaluation of CD10 expression and diagnosis of FL. Fine-needle aspiration biopsy/FCM (FNAB/FCM) could be a useful tool for verifying FL diagnosis and CD10 detection. Despite its heterogeneity, FL has a characteristic immunophenotype. BCL2R and BCL6R FL cases differ mainly in levels of BCL2 and CD10 with CD43 co-expression; BCL2(+)
Substances chimiques
BCL2 protein, human
0
BCL6 protein, human
0
Proto-Oncogene Proteins c-bcl-2
0
Proto-Oncogene Proteins c-bcl-6
0
Neprilysin
EC 3.4.24.11
Types de publication
Comparative Study
Evaluation Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
453-463Subventions
Organisme : Maria Sklodowska-Curie National Research Institute of Oncology
ID : GW23/2017
Organisme : Operational Programme Innovative Economy 2007-2013, Priority II. R&D Infrastructure, Measure 2.3
ID : POIG.02.03.00-14-111/13
Informations de copyright
© 2020 John Wiley & Sons Ltd.
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