Exogenous maltose enhances Zebrafish immunity to levofloxacin-resistant Vibrio alginolyticus.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
09
09
2019
revised:
09
04
2020
accepted:
10
04
2020
pubmed:
5
5
2020
medline:
15
5
2021
entrez:
5
5
2020
Statut:
ppublish
Résumé
Understanding the interplay between bacterial fitness, antibiotic resistance, host immunity and host metabolism could guide treatment and improve immunity against antibiotic-resistant pathogens. The acquisition of levofloxacin (Lev) resistance affects the fitness of Vibrio alginolyticus in vitro and in vivo. Lev-resistant (Lev-R) V. alginolyticus exhibits slow growth, reduced pathogenicity and greater resistance to killing by the host, Danio rerio (zebrafish), than Lev-sensitive (Lev-S) V. alginolyticus, suggesting that Lev-R V. alginolyticus triggers a weaker innate immune response in D. rerio than Lev-S V. alginolyticus. Differences were detected in the metabolome of D. rerio infected with Lev-S or Lev-R V. alginolyticus. Maltose, a crucial metabolite, is significantly downregulated in D. rerio infected with Lev-R V. alginolyticus, and exogenous maltose enhances the immune response of D. rerio to Lev-R V. alginolyticus, leading to better clearance of the infection. Furthermore, we demonstrate that exogenous maltose stimulates the host production of lysozyme and its binding to Lev-R V. alginolyticus, which depends on bacterial membrane potential. We suggest that exogenous exposure to crucial metabolites could be an effective strategy for treating and/or managing infections with antibiotic-resistant bacteria.
Identifiants
pubmed: 32364684
doi: 10.1111/1751-7915.13582
pmc: PMC7264874
doi:
Substances chimiques
Maltose
69-79-4
Levofloxacin
6GNT3Y5LMF
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1213-1227Informations de copyright
© 2020 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
Références
Clin Infect Dis. 2012 Jun;54 Suppl 5:S391-5
pubmed: 22572659
Proc Natl Acad Sci U S A. 2018 Feb 13;115(7):E1578-E1587
pubmed: 29382755
J Bacteriol. 2013 Jun;195(12):2755-67
pubmed: 23564175
Trends Biochem Sci. 2016 May;41(5):460-471
pubmed: 26935843
Fish Shellfish Immunol. 2019 Sep;92:508-518
pubmed: 31247319
Front Immunol. 2016 Dec 26;7:652
pubmed: 28082986
Mol Cell. 2008 Oct 10;32(1):129-39
pubmed: 18851839
Mol Cell Biol. 2003 Jun;23(12):4386-400
pubmed: 12773578
Fish Shellfish Immunol. 2016 May;52:31-43
pubmed: 26975410
Nutrients. 2017 Jun 17;9(6):
pubmed: 28629136
Biochemistry. 1993 May 4;32(17):4641-9
pubmed: 8485142
Fish Shellfish Immunol. 2014 May;38(1):15-24
pubmed: 24632045
J Pharmacol Toxicol Methods. 2008 May-Jun;57(3):176-87
pubmed: 18337127
Sci Immunol. 2017 Feb 10;2(8):
pubmed: 28386604
Dev Comp Immunol. 2004 Jun;28(7-8):747-54
pubmed: 15043943
J Evol Biol. 2014 Feb;27(2):303-12
pubmed: 24329755
J Food Prot. 2015 Nov;78(11):2089-92
pubmed: 26555534
Cell Mol Life Sci. 2011 Mar;68(6):1053-64
pubmed: 20734102
Nat Rev Microbiol. 2010 Apr;8(4):260-71
pubmed: 20208551
Genes Dev. 2000 Aug 15;14(16):2106-22
pubmed: 10950873
Metabolomics. 2007 Sep;3(3):211-221
pubmed: 24039616
Sci Transl Med. 2015 Jul 22;7(297):297ra114
pubmed: 26203082
J Insect Physiol. 2016 Feb;85:76-85
pubmed: 26585102
Curr Opin Microbiol. 2003 Oct;6(5):452-6
pubmed: 14572536
Semin Immunol. 2013 Apr;25(2):146-51
pubmed: 23757291
J Chromatogr A. 2019 May 10;1592:47-54
pubmed: 30685186
J Immunol Res. 2016;2016:6374379
pubmed: 28097157
J Bacteriol. 1998 Feb;180(4):831-9
pubmed: 9473036
J Proteome Res. 2016 Jul 1;15(7):2246-53
pubmed: 27251450
Semin Immunol. 2016 Oct;28(5):408-416
pubmed: 28340958
Fish Shellfish Immunol. 2020 Feb;97:359-366
pubmed: 31866447
Cell Rep. 2017 Jun 27;19(13):2743-2755
pubmed: 28658622
Lett Appl Microbiol. 2005;41(2):202-7
pubmed: 16033522
EXS. 1996;75:433-49
pubmed: 8765312
Biophys J. 2007 Jul 1;93(1):140-53
pubmed: 17434939
Clin Transl Immunology. 2016 Apr 22;5(4):e73
pubmed: 27195116
Curr Opin Struct Biol. 1996 Oct;6(5):679-91
pubmed: 8913692
Front Microbiol. 2016 Jun 15;7:843
pubmed: 27379026
Environ Microbiol. 2019 Dec;21(12):4724-4739
pubmed: 31595636
Science. 2014 Sep 26;345(6204):1250684
pubmed: 25258083
Emerg Microbes Infect. 2019;8(1):1229-1239
pubmed: 31448697
Nat Chem Biol. 2011 Dec 15;8(1):6-9
pubmed: 22173343
Nat Commun. 2019 Jul 25;10(1):3325
pubmed: 31346171
Front Immunol. 2017 Jun 13;8:611
pubmed: 28659912
Curr Opin Immunol. 2014 Oct;30:54-62
pubmed: 25064714
Protein Cell. 2015 Sep;6(9):628-37
pubmed: 26135925
J Proteome Res. 2015 Mar 6;14(3):1612-20
pubmed: 25675328
Environ Microbiol. 2019 Dec;21(12):4614-4628
pubmed: 31355499
Cell Metab. 2015 Feb 3;21(2):249-262
pubmed: 25651179
Development. 2017 May 15;144(10):1798-1806
pubmed: 28512197
Microorganisms. 2019 Jun 22;7(6):
pubmed: 31234491