Transcriptome analysis reveals rapid defence responses in wheat induced by phytotoxic aphid Schizaphis graminum feeding.
Animals
Aphids
/ physiology
Biosynthetic Pathways
/ genetics
Chlorophyll
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Host-Parasite Interactions
Hydrogen Peroxide
/ metabolism
Plant Diseases
/ genetics
Plant Immunity
/ genetics
Plant Leaves
/ genetics
Plant Proteins
/ genetics
Signal Transduction
/ genetics
Triticum
/ genetics
Chlorophyll content
Defence responses
Hydrogen peroxide accumulation
NADPH oxidase
Schizaphis graminum
Transcriptomics analysis
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
04 May 2020
04 May 2020
Historique:
received:
23
12
2019
accepted:
20
04
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
12
1
2021
Statut:
epublish
Résumé
Schizaphis graminum is one of the most important and devastating cereal aphids worldwide, and its feeding can cause chlorosis and necrosis in wheat. However, little information is available on the wheat defence responses triggered by S. graminum feeding at the molecular level. Here, we collected and analysed transcriptome sequencing data from leaf tissues of wheat infested with S. graminum at 2, 6, 12, 24 and 48 hpi (hours post infestation). A total of 44,835 genes were either up- or downregulated and differed significantly in response to aphid feeding. The expression levels of a number of genes (9761 genes) were significantly altered within 2 hpi and continued to change during the entire 48 h experiment. Gene Ontology analysis showed that the downregulated DEGs were mainly enriched in photosynthesis and light harvesting, and the total chlorophyll content in wheat leaves was also significantly reduced after S. graminum infestation at 24 and 48 hpi. However, a number of related genes of the salicylic acid (SA)-mediated defence signalling pathway and MAPK-WRKY pathway were significantly upregulated at early feeding time points (2 and 6 hpi). In addition, the gene expression and activity of antioxidant enzymes, such as peroxidase and superoxide dismutase, were rapidly increased at 2, 6 and 12 hpi. DAB staining results showed that S. graminum feeding induced hydrogen peroxide (H Our transcriptomic analysis revealed that defence-related pathways and oxidative stress in wheat were rapidly induced within hours after the initiation of aphid feeding. Additionally, NADPH oxidase plays an important role in aphid-induced defence responses and H
Sections du résumé
BACKGROUND
BACKGROUND
Schizaphis graminum is one of the most important and devastating cereal aphids worldwide, and its feeding can cause chlorosis and necrosis in wheat. However, little information is available on the wheat defence responses triggered by S. graminum feeding at the molecular level.
RESULTS
RESULTS
Here, we collected and analysed transcriptome sequencing data from leaf tissues of wheat infested with S. graminum at 2, 6, 12, 24 and 48 hpi (hours post infestation). A total of 44,835 genes were either up- or downregulated and differed significantly in response to aphid feeding. The expression levels of a number of genes (9761 genes) were significantly altered within 2 hpi and continued to change during the entire 48 h experiment. Gene Ontology analysis showed that the downregulated DEGs were mainly enriched in photosynthesis and light harvesting, and the total chlorophyll content in wheat leaves was also significantly reduced after S. graminum infestation at 24 and 48 hpi. However, a number of related genes of the salicylic acid (SA)-mediated defence signalling pathway and MAPK-WRKY pathway were significantly upregulated at early feeding time points (2 and 6 hpi). In addition, the gene expression and activity of antioxidant enzymes, such as peroxidase and superoxide dismutase, were rapidly increased at 2, 6 and 12 hpi. DAB staining results showed that S. graminum feeding induced hydrogen peroxide (H
CONCLUSIONS
CONCLUSIONS
Our transcriptomic analysis revealed that defence-related pathways and oxidative stress in wheat were rapidly induced within hours after the initiation of aphid feeding. Additionally, NADPH oxidase plays an important role in aphid-induced defence responses and H
Identifiants
pubmed: 32366323
doi: 10.1186/s12864-020-6743-5
pii: 10.1186/s12864-020-6743-5
pmc: PMC7199342
doi:
Substances chimiques
Plant Proteins
0
Chlorophyll
1406-65-1
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339Subventions
Organisme : National Natural Science Foundation of China
ID : 31901881
Organisme : National Natural Science Foundation of China
ID : 31871979
Organisme : National Key R&D Plan in China
ID : 2017YFD0201700
Organisme : National Key R&D Plan in China
ID : 2017YFD0200900
Organisme : National Key R&D Plan in China
ID : 2016YFD0300700
Organisme : State Modern Agricultural Industry Technology System
ID : CARS-22-G-18
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