Tumor-associated Macrophages Facilitate Bladder Cancer Progression by Increasing Cell Growth, Migration, Invasion and Cytokine Expression.
Cell Line, Tumor
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Chemokine CCL5
/ metabolism
Culture Media, Conditioned
/ pharmacology
Cytokines
/ metabolism
Disease Progression
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
Interleukin-8
/ genetics
Macrophages
/ drug effects
Neoplasm Invasiveness
Neoplasm Staging
Urinary Bladder Neoplasms
/ pathology
Tumor-associated macrophages
bladder cancer
cell growth
cell invasion
cell migration
cytokines
tumor microenvironment
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
May 2020
May 2020
Historique:
received:
16
01
2020
revised:
29
03
2020
accepted:
30
03
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
15
5
2020
Statut:
ppublish
Résumé
Interactions between stromal and tumor cells in tumor microenvironment contribute to tumor progression. In bladder cancer (BCa), infiltration of macrophages in tumors correlates with cancer progression. Herein, the aim was to study the paracrine effects of tumor-associated macrophages (TAM) on BCa cells. The correlation between TAMs and tumor grade and stages was examined in tumor tissue microarrays. In addition, a conditioned media (CM) model was employed to investigate the paracrine effects of macrophages on BCa cell growth, migration, and invasion, as well as on the cytokine profile of each cell line. The correlation of tumor-infiltrating macrophages with high-grade and muscle-invasive BCa was demonstrated in human bladder tumor tissue microarrays. CM from co-cultures of macrophages and BCa cells increased BCa cell growth, migration and invasion. Moreover, higher mRNA and protein expression levels of CCL5 and IL-8 were found in cells and CM from co-cultures, respectively. The paracrine interaction between BCa cells and TAMs led to enhanced BCa cell growth, migration, and invasiveness, and moreover, increased IL-8 and CCL5 cytokine production in tumor microenvironment.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
Interactions between stromal and tumor cells in tumor microenvironment contribute to tumor progression. In bladder cancer (BCa), infiltration of macrophages in tumors correlates with cancer progression. Herein, the aim was to study the paracrine effects of tumor-associated macrophages (TAM) on BCa cells.
MATERIALS AND METHODS
METHODS
The correlation between TAMs and tumor grade and stages was examined in tumor tissue microarrays. In addition, a conditioned media (CM) model was employed to investigate the paracrine effects of macrophages on BCa cell growth, migration, and invasion, as well as on the cytokine profile of each cell line.
RESULTS
RESULTS
The correlation of tumor-infiltrating macrophages with high-grade and muscle-invasive BCa was demonstrated in human bladder tumor tissue microarrays. CM from co-cultures of macrophages and BCa cells increased BCa cell growth, migration and invasion. Moreover, higher mRNA and protein expression levels of CCL5 and IL-8 were found in cells and CM from co-cultures, respectively.
CONCLUSION
CONCLUSIONS
The paracrine interaction between BCa cells and TAMs led to enhanced BCa cell growth, migration, and invasiveness, and moreover, increased IL-8 and CCL5 cytokine production in tumor microenvironment.
Identifiants
pubmed: 32366417
pii: 40/5/2715
doi: 10.21873/anticanres.14243
doi:
Substances chimiques
Chemokine CCL5
0
Culture Media, Conditioned
0
Cytokines
0
Interleukin-8
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2715-2724Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.