A Comparative Study of the Action of Protonophore Uncouplers and Decoupling Agents as Inducers of Free Respiration in Mitochondria in States 3 and 4: Theoretical and Experimental Approaches.
2,4-Dinitrophenol
/ chemistry
Adenosine Triphosphate
/ chemistry
Animals
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
/ chemistry
Cyclosporine
/ chemistry
Liver
/ metabolism
Membrane Potential, Mitochondrial
Mitochondria
/ metabolism
Mitochondria, Liver
/ metabolism
Oxygen
/ chemistry
Oxygen Consumption
Palmitic Acid
/ chemistry
Phosphorylation
Protons
Rats
Rats, Wistar
Chemiosmotic theory
Decoupling
Fatty acids
Mitochondria
Oxidative phosphorylation
Uncoupling
Journal
Cell biochemistry and biophysics
ISSN: 1559-0283
Titre abrégé: Cell Biochem Biophys
Pays: United States
ID NLM: 9701934
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
received:
02
01
2020
accepted:
17
04
2020
pubmed:
6
5
2020
medline:
30
1
2021
entrez:
6
5
2020
Statut:
ppublish
Résumé
Theoretical and experimental studies have revealed that that in the liver mitochondria an increase in the rate of free respiration in state 3 induced by protonophore uncouplers 2,4-dinitrophenol and сarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone is equal to or slightly greater than the increase in respiration rate in state 4 induced by these uncouplers. In contrast to these protonophore uncouplers, the decoupler α,ω-tetradecanedioic acid, increasing the rate of respiration in state 4, does not significantly affect the rate of free respiration in state 3. We have proposed quantitative indicators that allow determining the constituent part of the rate of respiration in state 4, associated with the decoupling effect of the uncoupler. Using the example of palmitic acid, we have found out the fundamental possibility of the simultaneous functioning of uncouplers by two mechanisms: as protonophores and as decouplers. The data obtained contradict the delocalized version of Mitchell's chemiosmotic theory, but are in complete agreement with its local version. It can be assumed that the F
Identifiants
pubmed: 32367259
doi: 10.1007/s12013-020-00914-5
pii: 10.1007/s12013-020-00914-5
doi:
Substances chimiques
Protons
0
Palmitic Acid
2V16EO95H1
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
370-86-5
Cyclosporine
83HN0GTJ6D
Adenosine Triphosphate
8L70Q75FXE
2,4-Dinitrophenol
Q13SKS21MN
Oxygen
S88TT14065
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
203-216Références
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