A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies.
Animals
Antibodies, Neutralizing
/ metabolism
Antibodies, Viral
/ blood
Antibody Specificity
Chlorocebus aethiops
Control Groups
Cross Reactions
Enzyme-Linked Immunosorbent Assay
/ methods
Humans
Neutralization Tests
/ methods
Serologic Tests
/ methods
Vero Cells
Viral Plaque Assay
/ methods
Zika Virus
/ immunology
Zika Virus Infection
/ blood
Flavivirus
Neutralizing antibodies
Plaque reduction
Serology
Tissue culture
Zika virus
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
11
3
2021
Statut:
ppublish
Résumé
Neutralizing antibodies against Zika virus (ZIKV) are a robust indicator of exposure to ZIKV. Here we describe the plaque reduction neutralization test (PRNT), which quantifies ZIKV-neutralizing antibodies (nAbs) by incubating infectious ZIKV with different dilutions of sera and applying the mixture to a susceptible Vero cell monolayer. After 4 days of incubation, neutral red dye is added to optimize the visualization of plaques caused by ZIKV cytopathic effect. Compared with controls, sera containing nAbs against ZIKV will demonstrate ≥90% reduction in the number of plaques observed. This protocol can be utilized for research applications and serological diagnostic testing with the use of additional controls.
Identifiants
pubmed: 32367358
doi: 10.1007/978-1-0716-0581-3_5
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM