Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA.
Animals
Brazil
Cells, Cultured
Cote d'Ivoire
Culicidae
Haplorhini
Humans
Molecular Diagnostic Techniques
/ methods
Nucleic Acid Amplification Techniques
/ methods
Real-Time Polymerase Chain Reaction
/ methods
Recombinases
/ metabolism
Reproducibility of Results
Senegal
Uganda
Zika Virus
/ genetics
Zika Virus Infection
/ diagnosis
Diagnostics
Isothermal amplification
RT-LAMP
RT-PCR
RT-RPA
Zika virus
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
11
3
2021
Statut:
ppublish
Résumé
Molecular detection of Zika virus (ZIKV) is a key element of outbreak management. Multiple PCR and isothermal ZIKV assays targeting different ZIKV sequences have been published. In this study, we compared a qRT-PCR, 2 RT-LAMP assays (based on different primer design approaches), and an RT-RPA for the detection of African and Asian/American lineages of ZIKV isolates from human, mosquito, and monkey. Results showed that RT-LAMP detected 100% of samples with a time threshold (Tt) of 18.01 ± 11.71 min while qRT-PCR detected 88.88% of samples with a Tt of 58.30 ± 16.58 min and RT-RPA 50% of samples with a Tt of 3.70 ± 0.44 min.
Identifiants
pubmed: 32367367
doi: 10.1007/978-1-0716-0581-3_14
doi:
Substances chimiques
Recombinases
0
Types de publication
Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM