Emergence of variant avian infectious bronchitis virus in India.
Infectious bronchitis virus
Phylogeny
Sequence
Spike protein 1 gene
Variant infectious bronchitis virus
Journal
Iranian journal of veterinary research
ISSN: 1728-1997
Titre abrégé: Iran J Vet Res
Pays: Iran
ID NLM: 101660030
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
6
5
2020
Statut:
ppublish
Résumé
Infectious bronchitis virus (IBV) is the etiological agent of an acute and highly contagious disease. Infectious bronchitis (IB) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. The continuous evolution of the spike protein (S1) of IBV is responsible for the prevalence of many serotypes/genotypes around the world. Multiple lineages of IBV strains have been detected in chicken flocks in India since 2003. To detect IBV genotypes prevalent in India. Organ samples from 20 IBV-positive flocks with variable clinical signs were used for the amplification of the Positive PCR amplicons were sequenced. Sequence analysis showed that 14 field isolates belonged to the GI-1 genetic lineage (Mass 41 serotype), two field isolates belonged to the GI-13 (UK 4/91 variant IBV strain), one field isolate grouped with GIII, GV, and GVI genetic lineage and three belonged to a variant genotype unique to India (GI-24). Phylogenetic analysis also showed a similar type of grouping within the field isolates. Among the fourteen GI-1 isolates, 12 were isolated between 2003 and 2006 and only two were isolated between 2009 and 2011. The two field isolates belonging to GI-13 were isolated in 2007, another one belonging to GIII, GV, and GVI was isolated in 2010 and three field isolates were not close to any reference IBV sequences isolated in 2006 (IND-TN-168-06), 2010 (IND-TN-280-10) and 2011 (IND-TN-290-11). A unique variant of IBV is emerging in India (GI-24). Our findings will have important implications for future vaccine intervention.
Sections du résumé
BACKGROUND
BACKGROUND
Infectious bronchitis virus (IBV) is the etiological agent of an acute and highly contagious disease. Infectious bronchitis (IB) affects chicken of all ages and poses major economic loses to the poultry industry worldwide. The continuous evolution of the spike protein (S1) of IBV is responsible for the prevalence of many serotypes/genotypes around the world. Multiple lineages of IBV strains have been detected in chicken flocks in India since 2003.
AIMS
OBJECTIVE
To detect IBV genotypes prevalent in India.
METHODS
METHODS
Organ samples from 20 IBV-positive flocks with variable clinical signs were used for the amplification of the
RESULTS
RESULTS
Positive PCR amplicons were sequenced. Sequence analysis showed that 14 field isolates belonged to the GI-1 genetic lineage (Mass 41 serotype), two field isolates belonged to the GI-13 (UK 4/91 variant IBV strain), one field isolate grouped with GIII, GV, and GVI genetic lineage and three belonged to a variant genotype unique to India (GI-24). Phylogenetic analysis also showed a similar type of grouping within the field isolates. Among the fourteen GI-1 isolates, 12 were isolated between 2003 and 2006 and only two were isolated between 2009 and 2011. The two field isolates belonging to GI-13 were isolated in 2007, another one belonging to GIII, GV, and GVI was isolated in 2010 and three field isolates were not close to any reference IBV sequences isolated in 2006 (IND-TN-168-06), 2010 (IND-TN-280-10) and 2011 (IND-TN-290-11).
CONCLUSION
CONCLUSIONS
A unique variant of IBV is emerging in India (GI-24). Our findings will have important implications for future vaccine intervention.
Types de publication
Journal Article
Langues
eng
Pagination
33-39Références
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