CircRNA 001418 Promoted Cell Growth and Metastasis of Bladder Carcinoma via EphA2 by miR-1297.
Apoptosis
/ genetics
Cell Line, Tumor
Cell Proliferation
/ genetics
Cytochromes c
/ metabolism
Fas-Associated Death Domain Protein
/ genetics
Gene Expression Regulation, Neoplastic
Humans
MicroRNAs
/ genetics
RNA, Circular
/ genetics
Receptor, EphA2
/ genetics
Signal Transduction
Urinary Bladder
/ metabolism
Urinary Bladder Neoplasms
/ genetics
Circ 001418
EPHA2
FADD
bladder carcinoma
cytochrome c
miR-1297
Journal
Current molecular pharmacology
ISSN: 1874-4702
Titre abrégé: Curr Mol Pharmacol
Pays: United Arab Emirates
ID NLM: 101467997
Informations de publication
Date de publication:
2021
2021
Historique:
received:
13
12
2019
revised:
11
03
2020
accepted:
23
03
2020
pubmed:
6
5
2020
medline:
22
12
2021
entrez:
6
5
2020
Statut:
ppublish
Résumé
Cancer is one of the major causes of human deaths at present. It is the leading cause of deaths in developed countries. Moreover, Circular RNAs (circRNAs) have been discovered to play important roles in tumor genesis and development and are abnormally expressed in bladder cancer . The present study aims to investigate the anti-cancer effects of circ 001418 on bladder carcinoma and its possible mechanism. Quantitative PCR (qPCR) and gene chip were used to measure the circ 001418 expression. Cell proliferation and transfer, apoptosis and caspase-8 and caspase-3 activity levels were measured using MTT, Transwell assay, Flow cytometry. Caspase-3 and 9 activity levels, EphA2, cytochrome c and FADD protein expression, were detected using Western blotting. The expression of circ 001418 was increased in patients with bladder carcinoma. Over-expression of circ 001418 promoted cell proliferation and transfer, and reduced apoptosis in vitro model of bladder carcinoma. Down-regulation of Circ 001418 inhibited cell proliferation and transfer, and induced apoptosis in vitro model of bladder carcinoma. Meanwhile, over-expression of circ 001418 induced EphA2 and cytochrome c protein expression, and suppressed FADD protein expression in vitro model of bladder carcinoma by the suppression of miR-1297. MiR-1297 reduced the pro-cancer effect of circ 001418 on apoptosis of bladder carcinoma. Results showed that circRNA 001418 promoted cell growth and metastasis of bladder carcinoma via EphA2 by miR-1297.
Sections du résumé
BACKGROUND
Cancer is one of the major causes of human deaths at present. It is the leading cause of deaths in developed countries. Moreover, Circular RNAs (circRNAs) have been discovered to play important roles in tumor genesis and development and are abnormally expressed in bladder cancer .
OBJECTIVE
The present study aims to investigate the anti-cancer effects of circ 001418 on bladder carcinoma and its possible mechanism.
METHODS
Quantitative PCR (qPCR) and gene chip were used to measure the circ 001418 expression. Cell proliferation and transfer, apoptosis and caspase-8 and caspase-3 activity levels were measured using MTT, Transwell assay, Flow cytometry. Caspase-3 and 9 activity levels, EphA2, cytochrome c and FADD protein expression, were detected using Western blotting.
RESULTS
The expression of circ 001418 was increased in patients with bladder carcinoma. Over-expression of circ 001418 promoted cell proliferation and transfer, and reduced apoptosis in vitro model of bladder carcinoma. Down-regulation of Circ 001418 inhibited cell proliferation and transfer, and induced apoptosis in vitro model of bladder carcinoma. Meanwhile, over-expression of circ 001418 induced EphA2 and cytochrome c protein expression, and suppressed FADD protein expression in vitro model of bladder carcinoma by the suppression of miR-1297. MiR-1297 reduced the pro-cancer effect of circ 001418 on apoptosis of bladder carcinoma.
CONCLUSION
Results showed that circRNA 001418 promoted cell growth and metastasis of bladder carcinoma via EphA2 by miR-1297.
Identifiants
pubmed: 32368989
pii: CMP-EPUB-106359
doi: 10.2174/1874467213666200505093815
doi:
Substances chimiques
FADD protein, human
0
Fas-Associated Death Domain Protein
0
MIRN1297 microRNA, human
0
MicroRNAs
0
RNA, Circular
0
Cytochromes c
9007-43-6
Receptor, EphA2
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
68-78Informations de copyright
Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.