Activation of c-Jun by human cytomegalovirus UL42 through JNK activation.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
24
01
2020
accepted:
17
04
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
6
8
2020
Statut:
epublish
Résumé
c-Jun is a major component of the AP-1 transactivator complex. In this report, we demonstrated that AP-1 was activated by the expression of UL42, a human cytomegalovirus-encoded membrane protein that has two PPXY (PY) motifs and a C-terminal transmembrane domain (TMD). Although UL42 interacts with Itch, an ubiquitin E3 ligase, through the PY motifs, UL42 phosphorylated c-Jun and c-Jun N-terminal kinase (JNK) in the absence of any interaction with Itch. Experiments using mutated versions of UL42 suggest the importance of the carboxyl half (a.a. 52-124) of UL42 for the activation of the JNK signaling, while C-terminal TMD alone is not sufficient. Thus, we hypothesize that UL42 plays a role in the activation of JNK signaling in HCMV-infected cells. (118 words).
Identifiants
pubmed: 32369499
doi: 10.1371/journal.pone.0232635
pii: PONE-D-20-02197
pmc: PMC7199950
doi:
Substances chimiques
JUN protein, human
0
Proto-Oncogene Proteins c-jun
0
Repressor Proteins
0
Viral Proteins
0
ITCH protein, human
EC 2.3.2.26
Ubiquitin-Protein Ligases
EC 2.3.2.27
JNK Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0232635Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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