PIWI-interacting RNAs are differentially expressed during cardiac differentiation of human pluripotent stem cells.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
27
11
2019
accepted:
20
04
2020
entrez:
6
5
2020
pubmed:
6
5
2020
medline:
31
7
2020
Statut:
epublish
Résumé
PIWI-interacting RNAs (piRNAs) are a class of non-coding RNAs initially thought to be restricted exclusively to germline cells. In recent years, accumulating evidence has demonstrated that piRNAs are actually expressed in pluripotent, neural, cardiac and even cancer cells. However, controversy remains around the existence and function of somatic piRNAs. Using small RNA-seq samples from H9 pluripotent cells differentiated to mesoderm progenitors and cardiomyocytes we identified the expression of 447 piRNA transcripts, of which 241 were detected in pluripotency, 218 in mesoderm and 171 in cardiac cells. The majority of them originated from the sense strand of protein coding and lncRNAs genes in all stages of differentiation, though no evidences of amplification loop (ping-pong) were found. Genes hosting piRNA transcripts in cardiac samples were related to critical biological processes in the heart, like contraction and cardiac muscle development. Our results indicate that these piRNAs might have a role in fine-tuning the expression of genes involved in differentiation of pluripotent cells to cardiomyocytes.
Identifiants
pubmed: 32369512
doi: 10.1371/journal.pone.0232715
pii: PONE-D-19-32943
pmc: PMC7199965
doi:
Substances chimiques
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0232715Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Sci Rep. 2018 May 23;8(1):8072
pubmed: 29795287
Cell Stem Cell. 2013 Jan 3;12(1):127-37
pubmed: 23168164
Cell Stem Cell. 2012 Jan 6;10(1):16-28
pubmed: 22226352
Nucleic Acids Res. 2014 Aug;42(14):8984-95
pubmed: 25038252
Science. 2006 Jul 21;313(5785):363-7
pubmed: 16778019
Cell. 2007 Mar 23;128(6):1089-103
pubmed: 17346786
Physiol Rev. 2016 Oct;96(4):1297-325
pubmed: 27535639
Biochem Biophys Res Commun. 2014 Mar 28;446(1):218-23
pubmed: 24602614
Int J Biochem Cell Biol. 2014 Oct;55:153-6
pubmed: 25220478
J Immunol. 2010 Aug 15;185(4):2009-12
pubmed: 20631304
Bioinformatics. 2010 Mar 15;26(6):841-2
pubmed: 20110278
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13742-7
pubmed: 20643952
Nucleic Acids Res. 2011 Aug;39(15):6596-607
pubmed: 21546553
Science. 2007 May 4;316(5825):744-7
pubmed: 17446352
Nucleic Acids Res. 2016 Jul 8;44(W1):W160-5
pubmed: 27079975
Int J Biochem Cell Biol. 2016 Jul;76:1-11
pubmed: 27131603
J Bioinform Comput Biol. 2005 Aug;3(4):965-88
pubmed: 16078370
Nat Rev Cardiol. 2015 Jul;12(7):415-25
pubmed: 25855606
Nucleic Acids Res. 2019 Jan 8;47(D1):D175-D180
pubmed: 30371818
Cell Physiol Biochem. 2017;44(1):1-20
pubmed: 29130960
Genes Dev. 2014 Jul 1;28(13):1410-28
pubmed: 24939875
Cell. 2006 Dec 15;127(6):1193-207
pubmed: 17174894
Commun Biol. 2018 Jan 22;1:2
pubmed: 30271890
Cell. 2016 Jul 14;166(2):451-467
pubmed: 27419872
Mol Cancer. 2016 Jan 15;15:5
pubmed: 26768585
Cell Stem Cell. 2008 Mar 6;2(3):195-6
pubmed: 18371442
Nucleic Acids Res. 2016 Jan 4;44(D1):D216-22
pubmed: 26553799
Nat Struct Mol Biol. 2012 Aug;19(8):773-81
pubmed: 22842725
Nature. 2010 Oct 28;467(7319):1128-32
pubmed: 20953170
Genes Dev. 2006 Aug 15;20(16):2214-22
pubmed: 16882972
Nucleic Acids Res. 2016 Apr 7;44(6):2898-908
pubmed: 26826711
Nucleic Acids Res. 2005 Nov 27;33(20):e179
pubmed: 16314309
J Clin Invest. 2001 Aug;108(3):407-14
pubmed: 11489934
Nature. 2009 Oct 29;461(7268):1296-9
pubmed: 19812547
Nature. 2014 Jan 16;505(7483):353-359
pubmed: 24429634
Nat Rev Genet. 2019 Feb;20(2):89-108
pubmed: 30446728
Cell. 2012 Apr 27;149(3):693-707
pubmed: 22541438
Phys Biol. 2019 Jan 18;16(2):021001
pubmed: 30560804
Nat Protoc. 2013 Jan;8(1):162-75
pubmed: 23257984
EMBO J. 2010 Oct 6;29(19):3219-21
pubmed: 20924396
Nat Rev Mol Cell Biol. 2011 Apr;12(4):246-58
pubmed: 21427766
RNA. 2007 Oct;13(10):1693-702
pubmed: 17698640
Curr Top Dev Biol. 2012;100:279-317
pubmed: 22449848
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Essays Biochem. 2013;54:39-52
pubmed: 23829526
Nat Rev Mol Cell Biol. 2014 Aug;15(8):509-24
pubmed: 25027649
Nucleic Acids Res. 2008 Dec;36(21):e143
pubmed: 18940866
Mol Biosyst. 2017 Feb 28;13(3):565-576
pubmed: 28127595
Circ Res. 2017 Aug 4;121(4):376-391
pubmed: 28663367
Development. 2014 Sep;141(18):3458-71
pubmed: 25183868
Genes Dev. 2012 Nov 1;26(21):2361-73
pubmed: 23124062
Bioinformatics. 2019 Jan 15;35(2):335-336
pubmed: 29985981
Nature. 2006 Jul 13;442(7099):199-202
pubmed: 16751776
Mol Neurobiol. 2018 Jan;55(1):483-494
pubmed: 27966078
Cell. 2009 May 1;137(3):522-35
pubmed: 19395010