Diagnostic Utility and Pathogenic Role of Circulating MicroRNAs in Vasospastic Angina.
diagnosis
microRNA
pathogenesis
vasospastic angina
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
02 May 2020
02 May 2020
Historique:
received:
02
04
2020
revised:
26
04
2020
accepted:
27
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
7
5
2020
Statut:
epublish
Résumé
We investigated the diagnostic value and pathophysiological role of circulating microRNA (miR) in vasospastic angina (VA). We enrolled patients who underwent coronary angiography for chest pain to explore the miR's diagnostic utility. In addition, we investigated the role of miRs in regulating endothelial nitric oxide synthase (eNOS) expression in human coronary artery endothelial cells (hCAECs). Among the 121 patients, 46 were diagnosed with VA (VA group), 26 with insignificant coronary lesions (ICL group), and 49 with atherothrombotic angina (AA group). The VA group showed a significantly higher expression of miR-17-5p, miR-92a-3p, and miR-126-3p than the ICL group. In contrast, miR-221-3p and miR-222-3p were upregulated in the AA group compared to the VA group, and all levels of miR-17-5p, miR-92a-3p, miR-126-3p, miR-145-5p, miR-221-3p, and miR-222-3p differed between the AA group and the ICL group. In the hCAECs, transfection with mimics (pre-miR) of miR-17-5p, miR-92a-3p, and miR-126-3p was associated with eNOS suppression. Additionally, transfection with inhibitors (anti-miR) of miR-92a-3p significantly rescued the eNOS suppression induced by lipopolysaccharide. In conclusion, the circulating miRs not only proved to have diagnostic utility, but also contributed to pathogenesis by eNOS regulation.
Identifiants
pubmed: 32370169
pii: jcm9051313
doi: 10.3390/jcm9051313
pmc: PMC7290712
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
J Vasc Res. 2012;49(5):447-60
pubmed: 22797777
Blood. 2013 Jan 3;121(1):226-36
pubmed: 23144172
J Am Heart Assoc. 2016 Aug 15;5(8):
pubmed: 27528406
J Am Coll Cardiol. 1996 Apr;27(5):1156-61
pubmed: 8609335
Arterioscler Thromb Vasc Biol. 2012 Apr;32(4):979-87
pubmed: 22267480
Int J Cardiol Heart Vasc. 2019 Feb 23;22:205-209
pubmed: 30963096
Cancer Sci. 2016 Mar;107(3):326-34
pubmed: 26749252
Circulation. 1999 Jun 8;99(22):2864-70
pubmed: 10359729
Circ J. 2006 Apr;70(4):409-13
pubmed: 16565556
Korean Circ J. 2018 Sep;48(9):767-777
pubmed: 30146803
Nature. 2009 Aug 6;460(7256):705-10
pubmed: 19578358
J Clin Invest. 2007 Sep;117(9):2369-76
pubmed: 17786230
Eur Heart J. 2018 Jan 7;39(2):119-177
pubmed: 28886621
Circulation. 2009 May 12;119(18):2531-4
pubmed: 19433770
Circ Res. 2010 Sep 3;107(5):677-84
pubmed: 20595655
Basic Res Cardiol. 2012 Sep;107(5):296
pubmed: 22890560
J Am Coll Cardiol. 1999 May;33(6):1442-52
pubmed: 10334407
Circulation. 1996 Aug 1;94(3):266-71
pubmed: 8759065
Mol Cell Endocrinol. 2013 Jul 15;374(1-2):125-9
pubmed: 23648338
Eur Heart J. 2016 Jan 14;37(3):267-315
pubmed: 26320110
Cardiovasc Res. 2015 Sep 1;107(4):522-33
pubmed: 25994172
Circulation. 2011 Nov 1;124(18):1936-44
pubmed: 21969012
Circulation. 2006 Apr 4;113(13):1708-14
pubmed: 16585403
Circ Res. 2005 Mar 18;96(5):e48-57
pubmed: 15718498
Arterioscler Thromb Vasc Biol. 2003 Dec;23(12):2209-14
pubmed: 14592852
Sci Rep. 2017 Apr 06;7:46031
pubmed: 28383055
J Am Coll Cardiol. 1989 Nov 15;14(6):1456-63
pubmed: 2809004
J Cell Mol Med. 2014 Mar;18(3):371-90
pubmed: 24533657
Circulation. 2011 Oct 18;124(16):1774-82
pubmed: 22007100
Eur J Heart Fail. 2018 Jun;20(6):1000-1010
pubmed: 29314582
Eur Heart J. 2013 Oct;34(38):2949-3003
pubmed: 23996286
Proc Natl Acad Sci U S A. 2002 Nov 26;99(24):15524-9
pubmed: 12434020
Am Heart J. 1985 Aug;110(2):300-10
pubmed: 4025107
Circulation. 2000 Mar 21;101(11):1319-23
pubmed: 10725293
Circ J. 2014;78(11):2779-801
pubmed: 25273915
Jpn Circ J. 2001 Feb;65(2):81-6
pubmed: 11216830
Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1516-21
pubmed: 18227515
Eur Heart J. 2020 Jan 14;41(3):407-477
pubmed: 31504439
Int J Med Sci. 2014 Aug 28;11(11):1161-71
pubmed: 25249785
Inflammation. 1981 Jun;5(2):115-26
pubmed: 6268539
J Pharmacol Sci. 2007 Jun;104(2):109-15
pubmed: 17538233
Circulation. 1989 Jul;80(1):1-9
pubmed: 2661051
J Clin Invest. 2002 Jul;110(2):203-8
pubmed: 12122112
J Am Coll Cardiol. 1996 Jan;27(1):45-52
pubmed: 8522709
J Am Heart Assoc. 2014 Oct 27;3(6):e001249
pubmed: 25349183
Circ Res. 2006 Mar 17;98(5):682-9
pubmed: 16456098
Sci Rep. 2018 Jan 15;8(1):761
pubmed: 29335599
PLoS One. 2014 Oct 13;9(10):e109650
pubmed: 25310838
Proc Natl Acad Sci U S A. 2008 Jul 29;105(30):10513-8
pubmed: 18663219
Cell. 2004 Jan 23;116(2):281-97
pubmed: 14744438
N Engl J Med. 1986 Oct 23;315(17):1046-51
pubmed: 3093861
Elife. 2015 Aug 12;4:
pubmed: 26267216