Combining native and 'omics' mass spectrometry to identify endogenous ligands bound to membrane proteins.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
27
09
2019
accepted:
25
03
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
19
8
2020
Statut:
ppublish
Résumé
Ligands bound to protein assemblies provide critical information for function, yet are often difficult to capture and define. Here we develop a top-down method, 'nativeomics', unifying 'omics' (lipidomics, proteomics, metabolomics) analysis with native mass spectrometry to identify ligands bound to membrane protein assemblies. By maintaining the link between proteins and ligands, we define the lipidome/metabolome in contact with membrane porins and a mitochondrial translocator to discover potential regulators of protein function.
Identifiants
pubmed: 32371966
doi: 10.1038/s41592-020-0821-0
pii: 10.1038/s41592-020-0821-0
pmc: PMC7332344
mid: EMS86661
doi:
Substances chimiques
Ligands
0
Lipids
0
Membrane Proteins
0
Proteome
0
Banques de données
figshare
['10.6084/m9.figshare.12021057.v1']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
505-508Subventions
Organisme : Wellcome Trust
ID : 104633
Pays : United Kingdom
Organisme : European Research Council
ID : 695511
Pays : International
Organisme : Medical Research Council
ID : MR/N020413/1
Pays : United Kingdom
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