Saliva proteomic patterns in patients with molar incisor hypomineralization.
Adolescent
Child
Cohort Studies
Complement Activation
Dental Caries
/ metabolism
Dental Enamel Hypoplasia
/ metabolism
Extracellular Matrix
/ metabolism
Female
Glutathione
/ metabolism
Heme
/ metabolism
Humans
Incisor
/ metabolism
Male
Mass Spectrometry
Molar
/ metabolism
Neutrophils
/ metabolism
Proteome
Proteomics
/ methods
Saliva
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
05 05 2020
05 05 2020
Historique:
received:
13
11
2019
accepted:
16
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
8
1
2021
Statut:
epublish
Résumé
Molar incisor hypomineralization (MIH) is an endemic pediatric disease with an unclear pathogenesis. Considering that saliva controls enamel remineralization and that MIH is associated with higher saliva flow rate, we hypothesized that the protein composition of saliva is linked to disease. To test this, we enrolled 5 children aged 6-14 years with MIH showing at least one hypersensitive molar and 5 caries-free children without hypomineralization. Saliva samples were subjected to proteomic analysis followed by protein classification in to biological pathways. Among 618 salivary proteins identified with high confidence, 88 proteins were identified exclusively in MIH patients and 16 proteins in healthy controls only. Biological pathway analysis classified these 88 patient-only proteins to neutrophil-mediated adaptive immunity, the activation of the classical pathway of complement activation, extracellular matrix degradation, heme scavenging as well as glutathione -and drug metabolism. The 16 controls-only proteins were associated with adaptive immunity related to platelet degranulation and the lysosome. This report suggests that the proteaneous composition of saliva is affected in MIH patients, reflecting a catabolic environment which is linked to inflammation.
Identifiants
pubmed: 32371984
doi: 10.1038/s41598-020-64614-z
pii: 10.1038/s41598-020-64614-z
pmc: PMC7200701
doi:
Substances chimiques
Proteome
0
Heme
42VZT0U6YR
Glutathione
GAN16C9B8O
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7560Références
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