Development of a Digital RT-PCR Method for Absolute Quantification of Bluetongue Virus in Field Samples.

Bluetongue Quantitative Real Time -RT- PCR RNA quantification Reoviridae droplet digital RT-PCR

Journal

Frontiers in veterinary science
ISSN: 2297-1769
Titre abrégé: Front Vet Sci
Pays: Switzerland
ID NLM: 101666658

Informations de publication

Date de publication:
2020
Historique:
received: 29 11 2019
accepted: 11 03 2020
entrez: 7 5 2020
pubmed: 7 5 2020
medline: 7 5 2020
Statut: epublish

Résumé

Bluetongue (BT) is a major Office International des Epizooties (OIE)-listed disease of wild and domestic ruminants caused by several serotypes of Bluetongue virus (BTV), a virus with a segmented dsRNA genome belonging to the family

Identifiants

pubmed: 32373633
doi: 10.3389/fvets.2020.00170
pmc: PMC7186476
doi:

Types de publication

Journal Article

Langues

eng

Pagination

170

Informations de copyright

Copyright © 2020 Rocchigiani, Tilocca, Portanti, Vodret, Bechere, Di Domenico, Savini, Lorusso and Puggioni.

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Auteurs

Angela M Rocchigiani (AM)

Department of Sanità Animale, Istituto Zooprofilattico Sperimentale Della Sardegna, Sassari, Italy.

Maria G Tilocca (MG)

Department of Sanità Animale, Istituto Zooprofilattico Sperimentale Della Sardegna, Sassari, Italy.

Ottavio Portanti (O)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale Abruzzo e Molise, Teramo, Italy.

Bruna Vodret (B)

Department of Sanità Animale, Istituto Zooprofilattico Sperimentale Della Sardegna, Sassari, Italy.

Roberto Bechere (R)

Department of Sanità Animale, Istituto Zooprofilattico Sperimentale Della Sardegna, Sassari, Italy.

Marco Di Domenico (M)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale Abruzzo e Molise, Teramo, Italy.

Giovanni Savini (G)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale Abruzzo e Molise, Teramo, Italy.

Alessio Lorusso (A)

OIE Reference Laboratory for Bluetongue, Istituto Zooprofilattico Sperimentale Abruzzo e Molise, Teramo, Italy.

Giantonella Puggioni (G)

Department of Sanità Animale, Istituto Zooprofilattico Sperimentale Della Sardegna, Sassari, Italy.

Classifications MeSH