Stabilizing DNAzymes through Encapsulation in a Metal-Organic Framework.

DNAzymes continuous-flow conditions metal-organic frameworks reactivation stabilization

Journal

Chemistry (Weinheim an der Bergstrasse, Germany)
ISSN: 1521-3765
Titre abrégé: Chemistry
Pays: Germany
ID NLM: 9513783

Informations de publication

Date de publication:
09 Oct 2020
Historique:
received: 02 05 2020
pubmed: 7 5 2020
medline: 11 2 2021
entrez: 7 5 2020
Statut: ppublish

Résumé

DNAzymes are a promising class of bioinspired catalyst; however, their structural instability limits their potential. Herein, a method to stabilize DNAzymes by encapsulating them in a metal-organic framework (MOF) host is reported. This biomimetic mineralization process makes DNAzymes active under a wider range of conditions. The concept is demonstrated by encapsulating hemin-G-quadruplex (Hemin-G4) into zeolitic imidazolate framework-90 (ZIF-90), which indeed increases the DNAzyme's structural stability. The stabilized DNAzymes show activities in the presence of Exonuclease I, organic solvents, or high temperature. Owing to its elevated stability and heterogeneous nature, it is possible to perform catalysis under continuous-flow conditions, and the DNAzyme can be reactivated in situ by introducing K

Identifiants

pubmed: 32374926
doi: 10.1002/chem.202002178
doi:

Substances chimiques

DNA, Catalytic 0
Metal-Organic Frameworks 0
Hemin 743LRP9S7N

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

12931-12935

Informations de copyright

© 2020 Wiley-VCH GmbH.

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Auteurs

Huiye Zhong (H)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P.R. China.

Wei-Shang Lo (WS)

Department of Chemistry, Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.

Tiantian Man (T)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P.R. China.

Benjamin P Williams (BP)

Department of Chemistry, Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.

Dan Li (D)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P.R. China.

Sheng-Yu Chen (SY)

School of Physical Science and Technology, ShanghaiTech University, 393 Middle Huaxia Road, Pudong, Shanghai, 201210, P.R. China.

Hao Pei (H)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P.R. China.

Li Li (L)

Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, 500 Dongchuan Road, Shanghai, 200241, P.R. China.

Chia-Kuang Tsung (CK)

Department of Chemistry, Merkert Chemistry Center, Boston College, 2609 Beacon Street, Chestnut Hill, MA, 02467, USA.

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