Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates.
BMP
EGFR
FGF2
brain organizer
glutamatergic neurons
human iPSC variation
inhibitory neurons
neural stem cell
neural transcriptional dynamics
neurogenesis
neuronal trajectory variation
patterning of the cortex
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
05 05 2020
05 05 2020
Historique:
received:
04
03
2019
revised:
22
10
2019
accepted:
10
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
4
6
2021
Statut:
ppublish
Résumé
Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and human in vitro NSCs and monkey brain sections to model the generation of cortical neuronal fates. We show that conserved signaling mechanisms regulate the acute transition from proliferative NSCs to committed glutamatergic excitatory neurons. As human telencephalic NSCs develop from pluripotency in vitro, they transition through organizer states that spatially pattern the cortex before generating glutamatergic precursor fates. NSCs derived from multiple human pluripotent lines vary in these early patterning states, leading differentially to dorsal or ventral telencephalic fates. This work furthers systematic analyses of the earliest patterning events that generate the major neuronal trajectories of the human telencephalon.
Identifiants
pubmed: 32375049
pii: S2211-1247(20)30548-9
doi: 10.1016/j.celrep.2020.107599
pmc: PMC7357345
mid: NIHMS1591508
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107599Subventions
Organisme : NEI NIH HHS
ID : R01 EY002593
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA006973
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH113257
Pays : United States
Organisme : NIDA NIH HHS
ID : R37 DA023999
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH109904
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA177669
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH110926
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH116488
Pays : United States
Organisme : NIMH NIH HHS
ID : P50 MH106934
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA212007
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH106874
Pays : United States
Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
Trends Neurosci. 2016 May;39(5):290-299
pubmed: 27032601
Genes Dev. 1996 Dec 15;10(24):3129-40
pubmed: 8985182
Nat Neurosci. 2012 Feb 05;15(3):477-86, S1
pubmed: 22306606
Neuron. 2016 Sep 21;91(6):1219-1227
pubmed: 27657449
BMC Genomics. 2012 May 01;13:160
pubmed: 22549044
Mol Cell Neurosci. 2001 Jun;17(6):1001-13
pubmed: 11414789
Nat Biotechnol. 2009 Mar;27(3):275-80
pubmed: 19252484
Science. 2000 Apr 14;288(5464):344-9
pubmed: 10764649
Nature. 2019 Oct;574(7778):418-422
pubmed: 31619793
J Neurosci. 1999 Aug 15;19(16):7077-88
pubmed: 10436062
Nature. 2016 Jul 13;535(7612):367-75
pubmed: 27409810
J Neurosci. 2007 Oct 10;27(41):11103-11
pubmed: 17928452
Bioinformatics. 2017 Jun 15;33(12):1892-1894
pubmed: 28174896
Nature. 2001 Feb 8;409(6821):714-20
pubmed: 11217860
Development. 2000 Nov;127(22):4993-5005
pubmed: 11044412
Nat Rev Neurosci. 2017 May;18(5):299-309
pubmed: 28381833
Curr Opin Cell Biol. 2012 Apr;24(2):269-76
pubmed: 22342580
Development. 2014 Jul;141(14):2855-65
pubmed: 24948604
Cell. 2013 Nov 21;155(5):1008-21
pubmed: 24267887
Nature. 2018 Jan 24;553(7689):437-445
pubmed: 29364288
Neuron. 2007 Oct 25;56(2):252-69
pubmed: 17964244
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7652-7
pubmed: 17442747
Science. 2005 Nov 4;310(5749):805-10
pubmed: 16272112
Cereb Cortex. 2008 Jun;18(6):1455-65
pubmed: 17947347
Cell. 2012 May 11;149(4):899-911
pubmed: 22579290
Bioinformatics. 2010 Nov 1;26(21):2792-3
pubmed: 20810601
Nat Rev Neurosci. 2006 Sep;7(9):687-96
pubmed: 16883309
Development. 2018 Jan 9;145(1):
pubmed: 29180570
Nat Med. 2003 Oct;9(10):1306-12
pubmed: 14502277
Science. 2018 Dec 14;362(6420):
pubmed: 30545855
Nat Med. 2010 Jun;16(6):662-4
pubmed: 20473307
Development. 2012 May;139(9):1535-46
pubmed: 22492350
Nature. 2002 Jul 4;418(6893):50-6
pubmed: 12077607
Development. 1998 Jun;125(12):2315-25
pubmed: 9584130
Development. 2006 May;133(9):1831-44
pubmed: 16613831
Nucleic Acids Res. 2014 Jan;42(Database issue):D756-63
pubmed: 24259432
Nat Commun. 2015 Nov 17;6:8896
pubmed: 26573335
J Neurosci. 1999 Dec 15;19(24):10877-85
pubmed: 10594069
Mech Dev. 1996 Sep;59(1):89-102
pubmed: 8892235
Nat Biotechnol. 2017 Feb;35(2):154-163
pubmed: 28112759
J Neurosci. 2003 Jul 23;23(16):6399-403
pubmed: 12878679
Nat Neurosci. 1999 Sep;2(9):848
pubmed: 10461229
Nat Med. 2018 Oct;24(10):1579-1589
pubmed: 30127392
Neuron. 2016 Sep 21;91(6):1260-1275
pubmed: 27657450
Cell. 2013 Nov 21;155(5):997-1007
pubmed: 24267886
Science. 1988 Jul 8;241(4862):170-6
pubmed: 3291116
Nat Neurosci. 2017 May;20(5):648-660
pubmed: 28319609
J Neurosci. 2013 Jun 26;33(26):10802-14
pubmed: 23804101
Nat Rev Neurosci. 2007 Jun;8(6):427-37
pubmed: 17514196
Neuron. 1997 Aug;19(2):251-67
pubmed: 9292717
Nat Neurosci. 2006 Jun;9(6):743-51
pubmed: 16680166
Cell. 2018 Jan 11;172(1-2):289-304.e18
pubmed: 29307494
Neuron. 2013 Oct 30;80(3):633-47
pubmed: 24183016
Science. 2012 Sep 14;337(6100):1301-3
pubmed: 22984058
Bioinformatics. 2020 Jun 1;36(11):3592-3593
pubmed: 32167521
Stem Cells. 2014 Mar;32(3):770-8
pubmed: 24155149
Stem Cells. 2013 Jun;31(6):1193-201
pubmed: 23417928
Annu Rev Neurosci. 2003;26:355-80
pubmed: 14527269
Science. 2008 Jan 18;319(5861):304-9
pubmed: 18202285
Nature. 2010 Mar 25;464(7288):554-561
pubmed: 20154730
Nat Neurosci. 2019 Feb;22(2):243-255
pubmed: 30617258
Genome Biol. 2019 Mar 22;20(1):63
pubmed: 30902100
Nat Neurosci. 2007 Oct;10(10):1277-86
pubmed: 17828260
Cell Stem Cell. 2008 Dec 4;3(6):670-80
pubmed: 19041783
Stem Cell Res. 2012 Nov;9(3):237-48
pubmed: 22910561
Neuron. 2006 Jul 20;51(2):187-99
pubmed: 16846854
Nat Neurosci. 2018 Aug;21(8):1117-1125
pubmed: 30050107
J Neurosci. 2005 Jul 13;25(28):6550-60
pubmed: 16014716
Methods Mol Biol. 2014;1101:87-112
pubmed: 24233779
Cell. 2015 Jul 16;162(2):375-390
pubmed: 26186191
Mol Psychiatry. 2015 Jun;20(6):703-17
pubmed: 25733313
Trends Genet. 2016 Mar;32(3):139-146
pubmed: 26830258
Development. 2000 Dec;127(24):5253-63
pubmed: 11076748
Nature. 2014 Apr 10;508(7495):199-206
pubmed: 24695229
Science. 2018 Dec 14;362(6420):
pubmed: 30545853
Nature. 1990 Oct 25;347(6295):762-5
pubmed: 2172829
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
J Neurosci. 2011 Oct 26;31(43):15604-17
pubmed: 22031906
Cell Syst. 2019 May 22;8(5):395-411.e8
pubmed: 31121116
J Neurosci. 1999 Dec 1;19(23):10383-9
pubmed: 10575035
Wiley Interdiscip Rev Dev Biol. 2017 Jan;6(1):
pubmed: 27865053
Cell Stem Cell. 2016 Sep 1;19(3):341-54
pubmed: 27476965
Science. 2018 Dec 14;362(6420):
pubmed: 30545857
Cell. 2013 Aug 1;154(3):518-29
pubmed: 23911319
Science. 2017 Dec 8;358(6368):1318-1323
pubmed: 29217575
Methods Mol Biol. 2008;438:31-8
pubmed: 18369747
Neuron. 2011 Mar 10;69(5):893-905
pubmed: 21382550
Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7089-7094
pubmed: 30894491
Nature. 2018 Aug;560(7718):372-376
pubmed: 30046109
Cell Syst. 2019 Apr 24;8(4):281-291.e9
pubmed: 30954476
Cell Stem Cell. 2017 Apr 6;20(4):518-532.e9
pubmed: 28017796
Nature. 1961 Nov 25;192:766-8
pubmed: 17533671
Nature. 1998 Jan 22;391(6665):357-62
pubmed: 9450748
J Neurosci. 2000 Jul 1;20(13):5012-23
pubmed: 10864959
J Neurosci. 1998 Nov 1;18(21):8853-62
pubmed: 9786991
Science. 1974 Feb 1;183(4123):425-7
pubmed: 4203022
J Neurosci. 2005 Jan 5;25(1):247-51
pubmed: 15634788
Neuron. 2001 Nov 20;32(4):591-604
pubmed: 11719201
J Neurosci. 2014 Jan 22;34(4):1420-31
pubmed: 24453331
Neuron. 2005 Mar 24;45(6):873-86
pubmed: 15797549
Exp Neurol. 2013 Jan;239:202-9
pubmed: 23099416
Nature. 2006 Aug 17;442(7104):823-6
pubmed: 16799564
Genes Dev. 2008 Sep 15;22(18):2479-84
pubmed: 18794345
Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10725-30
pubmed: 20498066
Science. 1999 Aug 6;285(5429):906-9
pubmed: 10436162
Cell. 2015 Sep 24;163(1):55-67
pubmed: 26406371
Annu Rev Neurosci. 2017 Jul 25;40:629-652
pubmed: 28661727
Science. 2018 Dec 14;362(6420):
pubmed: 30545854
J Neurosci. 2002 Feb 1;22(3):863-75
pubmed: 11826116
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Nature. 2015 Feb 19;518(7539):355-359
pubmed: 25533951
Nat Commun. 2015 Mar 23;6:6500
pubmed: 25799239
Mol Psychiatry. 2015 Mar;20(3):361-8
pubmed: 24686136
Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12770-5
pubmed: 22761314
Dev Growth Differ. 2009 Oct;51(8):687-98
pubmed: 19703209
EMBO J. 2017 May 15;36(10):1316-1329
pubmed: 28283582
Neuron. 2014 Jul 2;83(1):51-68
pubmed: 24991954
Mech Dev. 2001 Feb;100(2):165-75
pubmed: 11165475
Cell. 2015 Jun 18;161(7):1644-55
pubmed: 26091041
Science. 2016 Jun 10;352(6291):1326-1329
pubmed: 27284195
Cell Stem Cell. 2013 May 2;12(5):559-72
pubmed: 23642365
Nat Rev Neurosci. 2008 Feb;9(2):110-22
pubmed: 18209730
Mol Psychiatry. 2017 Jun;22(6):820-835
pubmed: 27378147
Cell Stem Cell. 2019 Jan 3;24(1):93-106.e6
pubmed: 30503143
Science. 2001 Nov 2;294(5544):1071-4
pubmed: 11567107