Mitochondrial DNA segregation and replication restrict the transmission of detrimental mutation.
Animals
Cell Respiration
/ genetics
DNA Replication
DNA, Mitochondrial
/ genetics
Drosophila melanogaster
/ cytology
Electron Transport
Electron Transport Chain Complex Proteins
/ genetics
Female
Gene Expression Regulation, Developmental
Genes, Mitochondrial
Genome, Mitochondrial
Male
Mitochondria
Mitochondrial Dynamics
Mutation
Oocytes
/ cytology
Oogenesis
/ genetics
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 07 2020
06 07 2020
Historique:
received:
22
05
2019
revised:
15
11
2019
accepted:
10
04
2020
entrez:
7
5
2020
pubmed:
7
5
2020
medline:
18
3
2021
Statut:
ppublish
Résumé
Although mitochondrial DNA (mtDNA) is prone to accumulate mutations and lacks conventional DNA repair mechanisms, deleterious mutations are exceedingly rare. How the transmission of detrimental mtDNA mutations is restricted through the maternal lineage is debated. Here, we demonstrate that mitochondrial fission, together with the lack of mtDNA replication, segregate mtDNA into individual organelles in the Drosophila early germarium. After mtDNA segregation, mtDNA transcription begins, which activates respiration. Mitochondria harboring wild-type genomes have functional electron transport chains and propagate more vigorously than mitochondria containing deleterious mutations in hetreoplasmic cells. Therefore, mtDNA expression acts as a stress test for the integrity of mitochondrial genomes and sets the stage for replication competition. Our observations support selective inheritance at the organelle level through a series of developmentally orchestrated mitochondrial processes. We also show that the Balbiani body has a minor role in mtDNA selective inheritance by supplying healthy mitochondria to the pole plasm. These two mechanisms may act synergistically to secure the transmission of functional mtDNA through Drosophila oogenesis.
Identifiants
pubmed: 32375181
pii: 151740
doi: 10.1083/jcb.201905160
pmc: PMC7337505
pii:
doi:
Substances chimiques
DNA, Mitochondrial
0
Electron Transport Chain Complex Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
This is a work of the U.S. Government and is not subject to copyright protection in the United States. Foreign copyrights may apply.
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