Cytoplasmic Increase in Hsp70 Protein: A Potential New Biomarker of Early Infiltration of Cutaneous Squamous Cell Carcinoma Arising from Actinic Keratosis.
actinic keratosis
cutaneous squamous cell carcinoma
cytoplasm
heat shock protein.
skin cancer
Journal
Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829
Informations de publication
Date de publication:
03 05 2020
03 05 2020
Historique:
received:
09
02
2020
revised:
10
04
2020
accepted:
15
04
2020
entrez:
8
5
2020
pubmed:
8
5
2020
medline:
8
5
2020
Statut:
epublish
Résumé
Cutaneous squamous skin cell carcinoma (SCC) is the second most frequent type of non-melanoma skin cancer and is the second leading cause of death by skin cancer in Caucasian populations. However, at present it is difficult to predict patients with poor SCC prognosis. To identify proteins with expression levels that could predict SCC infiltration in SCC arising from actinic keratosis (SCC-AK). A total of 20 biopsies from 20 different patients were studied; 10 were SCC-AK samples and 10 were taken from normal skin. Early infiltrated SCC-AK samples were selected based on histological examination, and to determine the expression of proteins, fresh skin samples were processed by two-dimensional electrophoresis. The expression levels of three proteins, namely alpha hemoglobin and heat shock proteins 27 and 70 (Hsp27 and Hsp70, respectively) were significantly increased in SCC-AK samples with respect to normal control skin. However, only the expression level of Hsp70 protein positively correlated with the level of SCC-AK dermis infiltration. Immunohistological examination suggested that increased expression of Hsp70 proteins seemed to mainly occur in the cytoplasm of keratinocytes. The increased cytoplasmic Hsp70 expression in SCC-AK was confirmed by Western blot experiments. Cytoplasmic expression of Hsp70 could be a potential biomarker of early infiltration of SCC arising from AK.
Sections du résumé
BACKGROUND
Cutaneous squamous skin cell carcinoma (SCC) is the second most frequent type of non-melanoma skin cancer and is the second leading cause of death by skin cancer in Caucasian populations. However, at present it is difficult to predict patients with poor SCC prognosis.
OBJECTIVE
To identify proteins with expression levels that could predict SCC infiltration in SCC arising from actinic keratosis (SCC-AK).
METHODS
A total of 20 biopsies from 20 different patients were studied; 10 were SCC-AK samples and 10 were taken from normal skin. Early infiltrated SCC-AK samples were selected based on histological examination, and to determine the expression of proteins, fresh skin samples were processed by two-dimensional electrophoresis.
RESULTS
The expression levels of three proteins, namely alpha hemoglobin and heat shock proteins 27 and 70 (Hsp27 and Hsp70, respectively) were significantly increased in SCC-AK samples with respect to normal control skin. However, only the expression level of Hsp70 protein positively correlated with the level of SCC-AK dermis infiltration. Immunohistological examination suggested that increased expression of Hsp70 proteins seemed to mainly occur in the cytoplasm of keratinocytes. The increased cytoplasmic Hsp70 expression in SCC-AK was confirmed by Western blot experiments.
CONCLUSION
Cytoplasmic expression of Hsp70 could be a potential biomarker of early infiltration of SCC arising from AK.
Identifiants
pubmed: 32375264
pii: cancers12051151
doi: 10.3390/cancers12051151
pmc: PMC7281259
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : undefined
ID : 9
Pays : International
Organisme : Universidad Alfonso X El Sabio
ID : FCS/2015/3
Pays : International
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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