Effect of dietary supplementation with yeast cell wall extracts on performance and gut response in broiler chickens.
Gut morphometry
Immuno-histochemistry
Saccharomyces cerevisiae
Transcriptomic analysis
Journal
Journal of animal science and biotechnology
ISSN: 1674-9782
Titre abrégé: J Anim Sci Biotechnol
Pays: England
ID NLM: 101581293
Informations de publication
Date de publication:
2020
2020
Historique:
received:
09
12
2019
accepted:
12
03
2020
entrez:
8
5
2020
pubmed:
8
5
2020
medline:
8
5
2020
Statut:
epublish
Résumé
The dietary supplementation of yeast cell wall extracts (YCW) has been found to reduce pathogenic bacteria load, promote immunoglobulin production, prevent diseases by pro-inflammatory responses, and alter gut microbiota composition. This study evaluated growth and slaughter results, health, gut morphology, immune status and gut transcriptome of 576 male chickens fed two diets, i.e. C (control) or Y (with 250-500 g/t of YCW fractions according to the growth period). At 21 and 42 d the jejunum of 12 chickens per diet were sampled and stained with hematoxylin/eosin for morphometric evaluation, with Alcian-PAS for goblet cells, and antibodies against CD3+ intraepithelial T-cells and CD45+ intraepithelial leukocytes. The jejunum sampled at 42 d were also used for whole-transcriptome profiling. Dietary YCW supplementation did not affect final live weight, whereas it decreased feed intake (114 to 111 g/d; The dietary YCW supplementation improved broiler growth performance, increased gut glycoconjugate secretion and reduced the inflammatory status together with differences in the gut transcriptome, which can be considered useful to improve animal welfare and health under the challenging conditions of intensive rearing systems in broiler chickens.
Sections du résumé
BACKGROUND
BACKGROUND
The dietary supplementation of yeast cell wall extracts (YCW) has been found to reduce pathogenic bacteria load, promote immunoglobulin production, prevent diseases by pro-inflammatory responses, and alter gut microbiota composition. This study evaluated growth and slaughter results, health, gut morphology, immune status and gut transcriptome of 576 male chickens fed two diets, i.e. C (control) or Y (with 250-500 g/t of YCW fractions according to the growth period). At 21 and 42 d the jejunum of 12 chickens per diet were sampled and stained with hematoxylin/eosin for morphometric evaluation, with Alcian-PAS for goblet cells, and antibodies against CD3+ intraepithelial T-cells and CD45+ intraepithelial leukocytes. The jejunum sampled at 42 d were also used for whole-transcriptome profiling.
RESULTS
RESULTS
Dietary YCW supplementation did not affect final live weight, whereas it decreased feed intake (114 to 111 g/d;
CONCLUSIONS
CONCLUSIONS
The dietary YCW supplementation improved broiler growth performance, increased gut glycoconjugate secretion and reduced the inflammatory status together with differences in the gut transcriptome, which can be considered useful to improve animal welfare and health under the challenging conditions of intensive rearing systems in broiler chickens.
Identifiants
pubmed: 32377338
doi: 10.1186/s40104-020-00448-z
pii: 448
pmc: PMC7193382
doi:
Types de publication
Journal Article
Langues
eng
Pagination
40Informations de copyright
© The Author(s) 2020.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
Références
Poult Sci. 2008 Jul;87(7):1377-84
pubmed: 18577619
Poult Sci. 2009 Nov;88(11):2262-72
pubmed: 19834074
Bioinformatics. 2012 Dec 15;28(24):3211-7
pubmed: 23071270
Am J Physiol Renal Physiol. 2005 Sep;289(3):F496-503
pubmed: 16093425
Poult Sci. 2018 Jul 1;97(7):2339-2346
pubmed: 29618086
Cell Mol Gastroenterol Hepatol. 2019;7(3):555-570
pubmed: 30553809
Nat Immunol. 2016 Aug;17(8):930-7
pubmed: 27322654
Br Poult Sci. 2011 Dec;52(6):694-703
pubmed: 22221235
Front Microbiol. 2015 Dec 01;6:1336
pubmed: 26648920
Poult Sci. 2018 Jan 1;97(1):203-210
pubmed: 29077905
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Anim Nutr. 2017 Dec;3(4):399-405
pubmed: 29767160
J Anim Physiol Anim Nutr (Berl). 2015 Oct;99(5):932-7
pubmed: 25939376
J Anim Physiol Anim Nutr (Berl). 2012 Apr;96(2):275-84
pubmed: 21535233
Poult Sci. 2017 Jul 1;96(7):2064-2073
pubmed: 28339726
Poult Sci. 2016 Jun 1;95(6):1332-40
pubmed: 26944975
Nat Immunol. 2017 Apr;18(4):442-455
pubmed: 28250425
Poult Sci. 2012 Sep;91(9):2241-54
pubmed: 22912459
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Genome Biol. 2014;15(12):550
pubmed: 25516281
Poult Sci. 2012 Sep;91(9):2164-72
pubmed: 22912450
Poult Sci. 2013 Jul;92(7):1764-73
pubmed: 23776263
Am J Physiol Heart Circ Physiol. 2007 Jul;293(1):H142-51
pubmed: 17322420
Poult Sci. 2018 Feb 1;97(2):477-484
pubmed: 29211897
Sci Rep. 2017 Aug 15;7(1):8174
pubmed: 28811552
Res Vet Sci. 1986 Jan;40(1):32-40
pubmed: 3704321
Poult Sci. 2016 Mar;95(3):507-17
pubmed: 26740130
PLoS One. 2015 May 29;10(5):e0127267
pubmed: 26024524
Exp Cell Res. 2011 Nov 15;317(19):2702-10
pubmed: 21978911
Poult Sci. 2007 Jun;86(6):1070-8
pubmed: 17495075
Arthritis Rheum. 2012 Dec;64(12):3877-85
pubmed: 22886597
Poult Sci. 2009 Mar;88(3):601-7
pubmed: 19211531
J Poult Sci. 2018;55(1):38-46
pubmed: 32055154