Human Papillomavirus Quasivirus Production and Infection of Primary Human Keratinocytes.
HPV
infection
keratinocyte
papillomavirus
quasivirus
virion
Journal
Current protocols in microbiology
ISSN: 1934-8533
Titre abrégé: Curr Protoc Microbiol
Pays: United States
ID NLM: 101257113
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
entrez:
8
5
2020
pubmed:
8
5
2020
medline:
28
4
2021
Statut:
ppublish
Résumé
This protocol describes the production of human papillomavirus (HPV)-derived quasiviruses. Quasiviruses are infectious particles that are produced in 293TT packaging cells and contain a complete viral genome. We describe methods for infection of primary human keratinocytes with HPV quasiviruses, as well as assays to measure early viral DNA replication and transcription. Published 2020. U.S. Government. Basic Protocol 1: Transfection, harvest, and isolation of HPV quasiviruses Alternate Protocol 1: Packaging HPV DNA replicated in 293TT cells Alternate Protocol 2: Production of higher-purity quasivirus using the "Ripcord" method Support Protocol 1: Production of HPV minicircles Support Protocol 2: Production of recircularized HPV genomes Support Protocol 3: Screening of fractions for viral proteins Support Protocol 4: Screening of fractions for viral DNA Support Protocol 5: Measuring viral titer Support Protocol 6: Quantitation of quasivirions Basic Protocol 2: Infection of primary human foreskin keratinocytes with quasivirus Basic Protocol 3: HPV quasivirus transcription assay Basic Protocol 4: HPV quasivirus replication assay.
Identifiants
pubmed: 32378811
doi: 10.1002/cpmc.101
pmc: PMC7263449
mid: NIHMS1591012
doi:
Substances chimiques
Viral Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e101Subventions
Organisme : NIAID NIH HHS
ID : T32 AI125186
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA AI000713
Pays : United States
Informations de copyright
Published 2020. This article is a US Government work and is in the public domain in the USA.
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