Identification of Ca-rich dense granules in human platelets using scanning transmission X-ray microscopy.


Journal

Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878

Informations de publication

Date de publication:
01 May 2020
Historique:
received: 16 09 2019
accepted: 26 02 2020
entrez: 9 5 2020
pubmed: 10 5 2020
medline: 9 2 2021
Statut: ppublish

Résumé

Whole-mount (WM) platelet preparation followed by transmission electron microscopy (TEM) observation is the standard method currently used to assess dense granule (DG) deficiency (DGD). However, due to the electron-density-based contrast mechanism in TEM, other granules such as α-granules might cause false DG detection. Here, scanning transmission X-ray microscopy (STXM) was used to identify DGs and minimize false DG detection of human platelets. STXM image stacks of human platelets were collected at the calcium (Ca) L

Identifiants

pubmed: 32381773
pii: S1600577520002702
doi: 10.1107/S1600577520002702
pmc: PMC7285678
doi:

Substances chimiques

Calcium SY7Q814VUP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

720-724

Subventions

Organisme : Ministry of Science, ICT and Future Planning
ID : 2017M3A9G8084539
Organisme : Ministry of Science, ICT and Future Planning
ID : 2017K1A3A7A09016394

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Auteurs

Tung X Trinh (TX)

Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.

Sook Jin Kwon (SJ)

Center for Next Generation Cytometry, Hanyang University, Seoul 04763, Republic of Korea.

Zayakhuu Gerelkhuu (Z)

Center for Next Generation Cytometry, Hanyang University, Seoul 04763, Republic of Korea.

Jang Sik Choi (JS)

Center for Next Generation Cytometry, Hanyang University, Seoul 04763, Republic of Korea.

Jaewoo Song (J)

Department of Laboratory Medicine, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea.

Tae Hyun Yoon (TH)

Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.

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Classifications MeSH