Solution scattering at the Life Science X-ray Scattering (LiX) beamline.
SAXS
SEC-SAXS
WAXS
automation
solution scattering
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 May 2020
01 May 2020
Historique:
received:
05
11
2019
accepted:
19
02
2020
entrez:
9
5
2020
pubmed:
10
5
2020
medline:
9
2
2021
Statut:
ppublish
Résumé
This work reports the instrumentation and software implementation at the Life Science X-ray Scattering (LiX) beamline at NSLS-II in support of biomolecular solution scattering. For automated static measurements, samples are stored in PCR tubes and grouped in 18-position sample holders. Unattended operations are enabled using a six-axis robot that exchanges sample holders between a storage box and a sample handler, transporting samples from the PCR tubes to the X-ray beam for scattering measurements. The storage box has a capacity of 20 sample holders. At full capacity, the measurements on all samples last for ∼9 h. For in-line size-exclusion chromatography, the beamline-control software coordinates with a commercial high-performance liquid chromatography (HPLC) system to measure multiple samples in batch mode. The beamline can switch between static and HPLC measurements instantaneously. In all measurements, the scattering data span a wide q-range of typically 0.006-3.2 Å
Identifiants
pubmed: 32381785
pii: S1600577520002362
doi: 10.1107/S1600577520002362
pmc: PMC7206542
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
804-812Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM133893
Pays : United States
Organisme : NIH HHS
ID : P41 GM111244
Pays : United States
Informations de copyright
open access.
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