Effect of oral consumption of capsules containing Lactobacillus paracasei LPC-S01 on the vaginal microbiota of healthy adult women: a randomized, placebo-controlled, double-blind crossover study.
Gardnerella
Lactobacillus
16S rRNA gene profiling
community state types
probiotic
vaginal microbiota
Journal
FEMS microbiology ecology
ISSN: 1574-6941
Titre abrégé: FEMS Microbiol Ecol
Pays: England
ID NLM: 8901229
Informations de publication
Date de publication:
01 06 2020
01 06 2020
Historique:
received:
06
10
2019
accepted:
11
05
2020
pubmed:
10
5
2020
medline:
26
11
2020
entrez:
9
5
2020
Statut:
ppublish
Résumé
Oral consumption of probiotics is practical and can be an effective solution to preserve vaginal eubiosis. Here, we studied the ability of orally administered Lactobacillus paracasei LPC-S01 (DSM 26760) to affect the composition of the vaginal microbiota and colonize the vaginal mucosa in nondiseased adult women. A total of 40 volunteers took oral probiotic (24 billion CFU) or placebo capsules daily for 4 weeks, and after a 4-week washout, they switched to placebo or probiotic capsules according to the crossover design. A total of 23 volunteers completed the study according to the protocol. Before and after capsule ingestion, vaginal swabs were collected for qPCR quantification to detect L. paracasei LPC-S01 and for 16S rRNA gene sequencing. Vaginal swabs were grouped according to their bacterial taxonomic structure into nine community state types (CSTs), four of which were dominated by lactobacilli. Lactobacillus paracasei LPC-S01 was detected in the vagina of two participants. Statistical modeling (including linear mixed-effects model analysis) demonstrated that daily intake of probiotic capsules reduced the relative abundance of Gardnerella spp. Quantitative PCR with Gardnerella vaginalis primers confirmed this result. Considering the pathogenic nature of G. vaginalis, these results suggest a potential positive effect of this probiotic capsule on the vaginal microbial ecosystem.
Identifiants
pubmed: 32383767
pii: 5834546
doi: 10.1093/femsec/fiaa084
pmc: PMC7261233
pii:
doi:
Substances chimiques
Capsules
0
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© FEMS 2020.
Références
Probiotics Antimicrob Proteins. 2015 Mar;7(1):38-44
pubmed: 25362524
Clin Infect Dis. 2000 Jun;30(6):901-7
pubmed: 10852812
Taiwan J Obstet Gynecol. 2016 Aug;55(4):515-8
pubmed: 27590374
J Pathog. 2015;2015:648134
pubmed: 26798518
Obstet Gynecol. 2012 Jul;120(1):145-51
pubmed: 22914403
Appl Microbiol Biotechnol. 2018 Jan;102(2):553-558
pubmed: 29177936
Anaerobe. 2006 Oct-Dec;12(5-6):274-5
pubmed: 16965925
PLoS One. 2015 Aug 12;10(8):e0135620
pubmed: 26266808
J Infect Dis. 2014 Aug 1;210(3):338-43
pubmed: 24511102
BMC Infect Dis. 2017 Jun 5;17(1):394
pubmed: 28583109
FEMS Immunol Med Microbiol. 2003 Mar 20;35(2):131-4
pubmed: 12628548
J Clin Gastroenterol. 2004 Jul;38(6 Suppl):S107-10
pubmed: 15220672
Clin Exp Immunol. 2007 Sep;149(3):470-9
pubmed: 17590176
J Clin Microbiol. 2002 Oct;40(10):3871-3
pubmed: 12354905
Twin Res Hum Genet. 2006 Dec;9(6):844-8
pubmed: 17254419
Front Microbiol. 2018 Aug 02;9:1720
pubmed: 30116228
Eur J Obstet Gynecol Reprod Biol. 2013 May;168(1):75-9
pubmed: 23395559
Sci Rep. 2014 May 30;4:5136
pubmed: 24875844
Trends Microbiol. 2017 Mar;25(3):182-191
pubmed: 27914761
PLoS One. 2014 Aug 15;9(8):e104511
pubmed: 25127240
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
J Med Life. 2013;6(4):434-6
pubmed: 24868256
Genome Biol. 2013 Jan 14;14(1):R1
pubmed: 23316946
J Urol. 1987 Aug;138(2):330-5
pubmed: 3599250
FEMS Immunol Med Microbiol. 2001 Dec;32(1):37-41
pubmed: 11750220
Res Microbiol. 2017 Nov - Dec;168(9-10):859-864
pubmed: 28257809
Integr Med (Encinitas). 2016 Aug;15(4):42-7
pubmed: 27574494
Nat Ecol Evol. 2016 Dec 05;1(1):15
pubmed: 28812567
Appl Environ Microbiol. 1998 Jul 1;64(7):2418-23
pubmed: 9647809
J Clin Microbiol. 2001 Sep;39(9):3282-9
pubmed: 11526164
Sex Transm Infect. 2018 Mar;94(2):117-123
pubmed: 28947665
Cell Host Microbe. 2017 Jan 11;21(1):97-105
pubmed: 28017660
Front Microbiol. 2018 Jun 15;9:1278
pubmed: 29963028
Can J Urol. 2013 Feb;20(1):6607-14
pubmed: 23433130
Front Microbiol. 2016 Dec 08;7:1936
pubmed: 28008325
J Clin Microbiol. 2007 Sep;45(9):3145
pubmed: 17652481
Anaerobe. 2017 Oct;47:145-156
pubmed: 28552417
Clin Microbiol Rev. 2016 Apr;29(2):223-38
pubmed: 26864580
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4680-7
pubmed: 20534435
Can J Microbiol. 2017 Apr;63(4):269-277
pubmed: 28177795
BMC Microbiol. 2012 Oct 18;12:236
pubmed: 23078375
Trends Microbiol. 2018 Jan;26(1):16-32
pubmed: 28844447
Curr Microbiol. 2016 Oct;73(4):483-90
pubmed: 27324341
J Bacteriol. 2011 Mar;193(5):1034-41
pubmed: 21169489
Am J Obstet Gynecol. 2016 Nov;215(5):608.e1-608.e7
pubmed: 27342046
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7952-7
pubmed: 15911771
Int J Mol Sci. 2013 Jun 05;14(6):12004-12
pubmed: 23739678
Appl Environ Microbiol. 2016 Sep 16;82(19):5850-9
pubmed: 27451450
Front Microbiol. 2015 Sep 15;6:952
pubmed: 26441886
Oral Microbiol Immunol. 2000 Aug;15(4):269-72
pubmed: 11154414
J Nutr. 2014 Nov;144(11):1787-96
pubmed: 25332478
Arch Gynecol Obstet. 2015 Oct;292(4):861-7
pubmed: 25855055
BMC Womens Health. 2015 Dec 03;15:115
pubmed: 26635090
Appl Environ Microbiol. 1993 Mar;59(3):695-700
pubmed: 7683183
Nat Ecol Evol. 2017 Jan 04;1(1):32
pubmed: 28812558
J Physiol. 2017 Jan 15;595(2):451-463
pubmed: 27373840
Ann N Y Acad Sci. 2011 Aug;1230:48-58
pubmed: 21824165
Microb Ecol Health Dis. 2015 Aug 14;26:27799
pubmed: 26282697
Obstet Gynecol. 2012 Dec;120(6):1306-13
pubmed: 23168754
Obstet Gynecol. 2017 Apr;129(4):643-654
pubmed: 28277350
Nat Commun. 2019 Mar 21;10(1):1305
pubmed: 30899005
Arch Gynecol Obstet. 2014 Jun;289(6):1225-34
pubmed: 24318276
Clin Microbiol Rev. 1998 Jan;11(1):81-120
pubmed: 9457430
J Infect Dis. 2014 Jun 15;209(12):1989-99
pubmed: 24403560
Nat Rev Gastroenterol Hepatol. 2014 Aug;11(8):506-14
pubmed: 24912386
J Infect Dis. 2015 Dec 15;212(12):1856-61
pubmed: 26080369
Can J Microbiol. 2009 Feb;55(2):133-8
pubmed: 19295645
Sci Rep. 2018 Feb 2;8(1):2307
pubmed: 29396486
mBio. 2013 Aug 06;4(4):
pubmed: 23919998