Sequential Ras/MAPK and PI3K/AKT/mTOR pathways recruitment drives basal extrusion in the prostate-like gland of Drosophila.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
08 05 2020
Historique:
received: 29 03 2016
accepted: 16 04 2020
entrez: 10 5 2020
pubmed: 10 5 2020
medline: 18 8 2020
Statut: epublish

Résumé

One of the most important but less understood step of epithelial tumourigenesis occurs when cells acquire the ability to leave their epithelial compartment. This phenomenon, described as basal epithelial cell extrusion (basal extrusion), represents the first step of tumour invasion. However, due to lack of adequate in vivo model, implication of emblematic signalling pathways such as Ras/Mitogen-Activated Protein Kinase (MAPK) and phosphoinositide 3 kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signalling pathways, is scarcely described in this phenomenon. We have developed a unique model of basal extrusion in the Drosophila accessory gland. There, we demonstrate that both Ras/MAPK and PI3K/AKT/mTOR pathways are necessary for basal extrusion. Furthermore, as in prostate cancer, we show that these pathways are co-activated. This occurs through set up of Epidermal Growth Factor Receptor (EGFR) and Insulin Receptor (InR) dependent autocrine loops, a phenomenon that, considering human data, could be relevant for prostate cancer.

Identifiants

pubmed: 32385236
doi: 10.1038/s41467-020-16123-w
pii: 10.1038/s41467-020-16123-w
pmc: PMC7210301
doi:

Substances chimiques

Drosophila Proteins 0
Proto-Oncogene Proteins c-akt EC 2.7.11.1
TOR Serine-Threonine Kinases EC 2.7.11.1
Mitogen-Activated Protein Kinases EC 2.7.11.24

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2300

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Auteurs

Amandine Rambur (A)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Corinne Lours-Calet (C)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Claude Beaudoin (C)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Julio Buñay (J)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Marine Vialat (M)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Vincent Mirouse (V)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.

Amalia Trousson (A)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Yoan Renaud (Y)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.

Jean-Marc A Lobaccaro (JA)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Silvère Baron (S)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Laurent Morel (L)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France.

Cyrille de Joussineau (C)

Université Clermont Auvergne, GReD, CNRS UMR 6293, INSERM U1103, 28 place Henri Dunant, BP38, 63001, Clermont-Ferrand, France. cyrille.de_joussineau@uca.fr.
Centre de Recherche en Nutrition Humaine d'Auvergne, 58 Boulevard Montalembert, 63009, Clermont-Ferrand, France. cyrille.de_joussineau@uca.fr.

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