Par complex cluster formation mediated by phase separation.

Animals COS Cells Cell Cycle Cell Differentiation Cell Survival Chlorocebus aethiops Drosophila Proteins / chemistry Drosophila melanogaster / cytology Green Fluorescent Proteins / metabolism HEK293 Cells Humans Larva / cytology Multiprotein Complexes / chemistry Neurons / cytology Protein Domains Protein Kinase C / metabolism Rats

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
08 05 2020
Historique:
received: 28 07 2019
accepted: 16 04 2020
entrez: 10 5 2020
pubmed: 10 5 2020
medline: 6 8 2020
Statut: epublish

Résumé

The evolutionarily conserved Par3/Par6/aPKC complex regulates the polarity establishment of diverse cell types and distinct polarity-driven functions. However, how the Par complex is concentrated beneath the membrane to initiate cell polarization remains unclear. Here we show that the Par complex exhibits cell cycle-dependent condensation in Drosophila neuroblasts, driven by liquid-liquid phase separation. The open conformation of Par3 undergoes autonomous phase separation likely due to its NTD-mediated oligomerization. Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation. aPKC can also be concentrated to the Par3N/Par6 condensates as a client. Interestingly, activated aPKC can disperse the Par3/Par6 condensates via phosphorylation of Par3. Perturbations of Par3/Par6 phase separation impair the establishment of apical-basal polarity during neuroblast asymmetric divisions and lead to defective lineage development. We propose that phase separation may be a common mechanism for localized cortical condensation of cell polarity complexes.

Identifiants

DOI: 10.1038/s41467-020-16135-6 PMID: 32385244 PMC: PMC7211019
pubmed: 32385244
doi: 10.1038/s41467-020-16135-6
pii: 10.1038/s41467-020-16135-6
pmc: PMC7211019
doi:

Substances chimiques

Drosophila Proteins 0
Multiprotein Complexes 0
Green Fluorescent Proteins 147336-22-9
Protein Kinase C EC 2.7.11.13

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2266

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Auteurs

Ziheng Liu (Z)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Ying Yang (Y)

Temasek Life Sciences Laboratory, Department of Biological Sciences, National University of Singapore, Singapore, 117604, Singapore.

Aihong Gu (A)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Jiawen Xu (J)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Ying Mao (Y)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Haojie Lu (H)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.

Weiguo Hu (W)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China.
Fudan University Shanghai Cancer Center and Cancer Metabolism Laboratory, Fudan University, Shanghai, 200032, China.
ORCID: 0000-0002-7397-6800

Qun-Ying Lei (QY)

Fudan University Shanghai Cancer Center and Cancer Metabolism Laboratory, Fudan University, Shanghai, 200032, China.
ORCID: 0000-0002-8547-8518

Zhouhua Li (Z)

College of Life Sciences, Capital Normal University, Beijing, 100048, China.
ORCID: 0000-0002-7660-8579

Mingjie Zhang (M)

Division of Life Science, State Key Laboratory of Molecular Neuroscience, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
ORCID: 0000-0001-9404-0190

Yu Cai (Y)

Temasek Life Sciences Laboratory, Department of Biological Sciences, National University of Singapore, Singapore, 117604, Singapore. caiyu@tll.org.sg.
ORCID: 0000-0003-2044-3290

Wenyu Wen (W)

Department of Neurosurgery, Huashan Hospital, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, Ministry of Science and Technology, Institutes of Biomedical Sciences, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China. wywen@fudan.edu.cn.
ORCID: 0000-0002-0798-4730

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Par complex cluster formation mediated by...
questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Fibroblastes › Cellules COS › Par complex cluster formation mediated by...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Par complex cluster formation mediated by...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Par complex cluster formation mediated by...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Survie cellulaire › Par complex cluster formation mediated by...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Cercopithecidae › Cercopithecinae › Cercopithecus › Cercopithecus aethiops › Par complex cluster formation mediated by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Protéines de Drosophila › Par complex cluster formation mediated by...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › Drosophila melanogaster › Par complex cluster formation mediated by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines luminescentes › Protéines à fluorescence verte › Par complex cluster formation mediated by...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Par complex cluster formation mediated by...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Par complex cluster formation mediated by...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › Morphogenèse › Métamorphose biologique › Étapes du cycle de vie › Larve › Par complex cluster formation mediated by...
questionsmedicales.fr › Structures macromoléculaires › Complexes multiprotéiques › Par complex cluster formation mediated by...
questionsmedicales.fr › Cellules › Neurones › Par complex cluster formation mediated by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Par complex cluster formation mediated by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protein-Serine-Threonine Kinases › Protéine kinase C › Par complex cluster formation mediated by...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Par complex cluster formation mediated by...