Distinct iron homeostasis in C57BL/6 and Balb/c mouse strains.
ferroportin
hepcidin
hypoferremia of inflammation
innate immunity
labile iron pool
Journal
Physiological reports
ISSN: 2051-817X
Titre abrégé: Physiol Rep
Pays: United States
ID NLM: 101607800
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
22
03
2020
revised:
18
04
2020
accepted:
20
04
2020
entrez:
10
5
2020
pubmed:
10
5
2020
medline:
4
6
2021
Statut:
ppublish
Résumé
C57BL/6 (BL6) and Balb/c mice exhibit prototypical Th1- and Th2-dominant immune predispositions, respectively. Iron is a proinflammatory metal ion; however, limited information is documented on the differences in iron homeostasis between BL6 and Balb/c strains. The objective of this study was to investigate the extent to which strain-level differences in these mice dictates the regulation of iron homeostasis during physiologic and inflammatory conditions. At basal levels, Balb/c mice displayed significantly higher levels of iron in systemic circulation and tissue compared to BL6 mice. Moreover, Balb/c mice had greater iron absorption as indicated by higher gene expressions of duodenal DcytB, DMT1, Fpn, SFT, and Heph. Similarly, hepatic Tf, TfR1, TfR2, and DMT1 expressions were augmented in Balb/c mice. Interestingly, there was no change in hepatic Hamp expression between the two strains, suggesting that the disparity in their maintenance of iron is independent of hepcidin. Additionally, the basal levels of intracellular labile iron pool in Balb/c intestinal epithelial cells, and bone marrow-derived macrophages and neutrophils, were higher compared to BL6 mice. When mice were challenged with lipopolysaccharide, the acute inflammatory response in BL6 mice was more pronounced than in Balb/c mice, as indicated by the more rapid development of hypoferremia and upregulation of serum IL-6 and TNF-α levels in BL6 mice. In conclusion, this study underscores that iron homeostasis is distinct between BL6 and Balb/c strains under both physiologic and inflammatory conditions.
Identifiants
pubmed: 32385968
doi: 10.14814/phy2.14441
pmc: PMC7210116
doi:
Substances chimiques
Interleukin-6
0
Lipopolysaccharides
0
Tumor Necrosis Factor-alpha
0
interleukin-6, mouse
0
Iron
E1UOL152H7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14441Subventions
Organisme : NCI NIH HHS
ID : R01 CA219144
Pays : United States
Informations de copyright
© 2020 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.
Références
Blood. 2007 Jan 15;109(2):811-8
pubmed: 17003376
Methods Enzymol. 1978;52:302-10
pubmed: 672633
Blood. 1987 Oct;70(4):909-14
pubmed: 3651609
J Interferon Cytokine Res. 2006 Sep;26(9):682-8
pubmed: 16978073
Eur J Immunol. 2010 Mar;40(3):824-35
pubmed: 20039303
J Exp Med. 1994 Sep 1;180(3):969-76
pubmed: 7520477
Am J Physiol Gastrointest Liver Physiol. 2005 Jun;288(6):G1328-38
pubmed: 15637179
FEBS Lett. 1995 Feb 13;359(2-3):126-8
pubmed: 7867783
Hum Reprod. 2006 Nov;21(11):2810-6
pubmed: 16849816
Biometals. 2016 Jun;29(3):451-65
pubmed: 27007712
PLoS One. 2012;7(9):e44328
pubmed: 22957064
J Immunol. 2008 Aug 15;181(4):2723-31
pubmed: 18684963
J Leukoc Biol. 2009 Nov;86(5):1247-58
pubmed: 19652026
Blood. 2010 Apr 22;115(16):3374-81
pubmed: 20177050
J Clin Pathol. 1992 Feb;45(2):151-4
pubmed: 1541696
Biometals. 2015 Aug;28(4):733-43
pubmed: 26041486
Immunol Res. 2011 May;50(1):1-9
pubmed: 21161695
J Cell Sci. 2014 Oct 1;127(Pt 19):4279-91
pubmed: 25074810
Front Immunol. 2014 Nov 26;5:603
pubmed: 25505468
PLoS One. 2018 May 17;13(5):e0196921
pubmed: 29771935
Vet Pathol. 2012 Jan;49(1):32-43
pubmed: 22135019
Biochim Biophys Acta. 2002 Oct 2;1603(1):31-46
pubmed: 12242109
Exp Gerontol. 2009 Sep;44(9):594-600
pubmed: 19563877
PLoS One. 2015 Jan 28;10(1):e0117435
pubmed: 25629408
Biochim Biophys Acta. 1991 Sep 24;1094(3):288-91
pubmed: 1911880
Shock. 2004 Nov;22(5):460-6
pubmed: 15489639
Immunity. 2015 Sep 15;43(3):527-40
pubmed: 26362264
Eur J Nutr. 2013 Feb;52(1):135-43
pubmed: 22241739
J Nutr. 2001 Feb;131(2S-2):616S-633S; discussion 633S-635S
pubmed: 11160594
Clin Sci (Lond). 2001 Mar;100(3):239-47
pubmed: 11222109
Cell Metab. 2015 Nov 3;22(5):777-87
pubmed: 26437604
Gut. 2002 Nov;51(5):648-53
pubmed: 12377801
Biochem J. 2007 Apr 15;403(2):261-6
pubmed: 17233627
Blood Cells Mol Dis. 2003 Sep-Oct;31(2):256-61
pubmed: 12972034
Am J Clin Nutr. 2004 Mar;79(3):516-21
pubmed: 14985230
Carcinogenesis. 2006 Jan;27(1):162-9
pubmed: 16081511
J Leukoc Biol. 2003 Aug;74(2):287-94
pubmed: 12885946
FASEB J. 2016 Jan;30(1):252-61
pubmed: 26370847
Front Pharmacol. 2014 Jul 10;5:156
pubmed: 25071575
J Immunol. 2000 Jun 15;164(12):6166-73
pubmed: 10843666
J Immunol. 2012 Aug 15;189(4):1911-9
pubmed: 22786765
Physiol Rep. 2020 May;8(9):e14441
pubmed: 32385968
J Vis Exp. 2013 Jul 10;(77):e50586
pubmed: 23892876
CSH Protoc. 2008 Dec 01;2008:pdb.prot5080
pubmed: 21356739
Cell Microbiol. 2010 Dec;12(12):1691-702
pubmed: 20964797
J Biol Chem. 2017 Aug 4;292(31):12727-12734
pubmed: 28615456
J Infect Dis. 1997 Jun;175(6):1467-76
pubmed: 9180188
S Afr J Med Sci. 1968 Apr;33(1):9-11
pubmed: 5676884
J Nutr. 2003 May;133(5 Suppl 1):1468S-72S
pubmed: 12730445
Science. 2018 Mar 30;359(6383):1520-1523
pubmed: 29599243
PLoS One. 2013 Apr 23;8(4):e61050
pubmed: 23637785
Blood. 2003 May 15;101(10):4148-54
pubmed: 12522003
Cell Microbiol. 2007 Sep;9(9):2126-40
pubmed: 17466014
Annu Rev Nutr. 2006;26:323-42
pubmed: 16848710
J Clin Invest. 2004 May;113(9):1271-6
pubmed: 15124018